Protein Purification Reagents and Kits

Protein Purification Reagents and Kits
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Thermo Scientific™ Mitochondria Isolation Kit for Cultured Cells
Isolate intact mitochondria from cultured mammalian cell samples in about 40 minutes with this versatile, benchtop microcentrifuge-based fractionation kit.

Thermo Scientific™ HisPur™ Ni-NTA Chromatography Cartridges, 1 mL
Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.
Stationary Phase | Divalent Nickel, Metal Chelate |
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Type | Chromatography Cartridge |
Target | His-Tagged Protein |
Content And Storage | Store between 4°C and 8°C. Do not freeze. |
Column Type | Affinity, Agarose Resin |
Product Line | HisPur™ |
Thermo Scientific™ Subcellular Protein Fractionation Kit for Cultured Cells
Enrich for cultured cell-specific cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins in less than 3 hours.

Thermo Scientific™ Pierce™ Anti-DYKDDDDK Magnetic Agarose
Thermo Scientific™ Pierce™ Anti-DYKDDDK Magnetic Agarose Beads provide a fast, convenient method for purification of DYKDDDK-tagged recombinant proteins

dimMatrix | Magnetic Agarose |
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Thermo Scientific™ NE-PER™ Nuclear and Cytoplasmic Extraction Reagents
Efficiently lyse cells and extract separate cytoplasmic and nuclear protein fractions in less than two hours with this subcellular fractionation kit.

Buffer | Lysis Buffer |
---|---|
Shipping Condition | Ambient |
Form | Liquid |
Product Type | Nuclear and Cytoplasmic Extraction Reagent |
Reagent Type | Cell Lysis Buffer, Detergent Solution, Subcellular Fractionation |
Product Line | NE-PER™ |
Thermo Scientific™ Pierce™ 3x DYKDDDDK Peptide
Tricosa-peptide for competitively eluting DYKDDDK-tagged fusion proteins from immobilized supports

Shipping Condition | Wet Ice |
---|---|
Format | Lyophilized Powder |
Form | Lyophilized Powder |
Protein Form | Recombinant |
Product Type | Peptides |
dimMatrix | Affinity Resin |
For Use With (Application) | Protein Purification and Isolation |
Product Line | Pierce™ |
Thermo Scientific™ HisPur™ Ni-NTA Spin Plates
Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.
Thermo Scientific™ HisPur™ Cobalt Resin
Gently purify His-tagged recombinant fusion proteins using cobalt (Co2+) immobilized metal chelate affinity (IMAC) beads, spin columns and plates.
Stationary Phase | Metal Chelate, Divalent Cobalt |
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Type | Resin |
Target | His-Tagged Protein |
Content And Storage | Store at 4°C |
Format | Bottle |
Protein Form | Recombinant |
Form | Liquid Suspension |
Column Type | Affinity, Agarose Resin |
Product Line | HisPur™ |
Thermo Scientific™ HisPur™ Ni-NTA Spin Columns, 1 mL
Purify His-tagged recombinant fusion proteins using high-capacity nickel-NTA (nitrilotriacetic acid) metal-chelate beads, spin columns and plates.
Thermo Scientific™ AminoLink™ Plus Immobilization Kit, 2 mL
Covalently couple antibodies and other proteins via primary amines (-NH2) for affinity purification with these aldehyde-activated agarose beads.
Stationary Phase | Aldehyde |
---|---|
Type | Immobilization Kit |
Target | Antibody, Proteins through Primary Amine |
Format | Spin Column |
Column Type | Affinity, Agarose Resin |
Capacity (Metric) | 2 to 40 mg of protein Immobilization per Column |
Sufficient For | 2 to 40 mg of Protein per Column |
Product Line | AminoLink™ |
Thermo Scientific™ Pierce™ Glutathione Agarose
Purify GST-tagged recombinant fusion proteins from cell lysates using glutathione (GSH) beaded agarose affinity purification resins, spin columns and plates.
Stationary Phase | Glutathione |
---|---|
Type | Agarose |
Content And Storage | Upon receipt store at 4°C. |
Format | Bottle |
Protein Form | Recombinant |
Column Type | Affinity, Agarose Resin |
Capacity (Metric) | 40 mg Purified Recombinant GST Protein Binding per mL Resin |
Product Line | Pierce™ |
Thermo Scientific™ HisPur™ Ni-NTA Superflow Agarose
Perform FPLC purification of poly-histidine-tagged proteins with this nitrilotriacetic acid (NTA) modified Superflow 6 support charged with divalent nickel (Ni+2).
Stationary Phase | Divalent Nickel, Metal Chelate |
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Type | Agarose |
Target | His-Tagged Protein |
Content And Storage | Store at 4°C |
Format | Bottle |
Form | Liquid Suspension |
Column Type | Affinity, Agarose Resin |
Capacity (Metric) | 20 mg His-Tagged GFP Binding per mL Resin |
Product Line | HisPur™ |
Thermo Scientific™ Pierce™ NHS-Activated Agarose, Dry
N-Hydroxysuccinimide ester resin and columns to covalently immobilize proteins, peptides and other amine-containing ligands for affinity purification.
Stationary Phase | NHS Ester |
---|---|
Type | Agarose |
Target | Antibody, Protein, Peptide, Ligand |
Content And Storage | Upon receipt store dry resin at -20°C. Product is shipped in an ice pack. |
Format | Vial |
Protein Form | Recombinant |
Column Type | Affinity, Agarose Resin |
Capacity (Metric) | ≥25 mg per mL Coupling |
Product Line | Pierce™ |
Invitrogen™ Ni-NTA Agarose
Nickel-charged affinity resin that can be used to purify recombinant proteins containing a polyhistidine (6xHis) sequence
Type | Agarose |
---|---|
Stationary Phase | Ni-NTA |
Content And Storage | The Ni-NTA resin is pre-charged and able to bind up to 50 mg of recombinant protein per 1 ml of resin. It is provided as a 50% slurry in 30% ethanol. The resin will appear blue in color when charged with Ni2+ . Store at +4°C. Ni-NTA resin is guaranteed stable for 6 months when properly stored. |
Form | Suspension |
Column Type | Affinity Column |
Thermo Scientific™ AminoLink™ Coupling Resin
Covalently immobilize proteins and antibodies by lysine residues or other primary amines (-NH2) via reductive amination with these aldehyde-activated agarose beads.
Stationary Phase | Aldehyde |
---|---|
Type | Resin |
Target | Antibody, Proteins through Primary Amine |
Content And Storage | Store at 4°C. |
Format | Bottle |
Column Type | Affinity, Agarose Resin |
Product Line | AminoLink™ |