Enrich for cultured cell-specific cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins in less than 3 hours.
Brand: Thermo Scientific™ 78840
438.68 EUR valid until 2025-03-29
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Product Code. 10455924
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Perform segregation and enrichment of proteins from five different cellular compartments with Thermo Scientific™ Subcellular Protein Fractionation Kit for Cultured Cells.
The kit enables the stepwise separation and extraction of cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins from mammalian cultured cells. Extracts obtained with the Subcellular Protein Fractionation Kit for Cultured Cells are compatible with a variety of downstream applications including Western blotting, protein assays, electrophoretic mobility shift assays and reporter-gene and enzyme-activity assays. Fractionation of subcellular proteins enables protein localization assessment and protein enrichment from specific cellular compartments. The Thermo Scientific Subcellular Protein Fractionation Kit includes a combination of reagents for stepwise lysis of cells into functional cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal protein fractions in less than 3 hours. Extracts from each subcellular compartment generally have less than 15% contamination between fractions, which is sufficient purity for most experiments studying protein localization and redistribution.Highlights:
Concise – extract functional cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal protein fractions in less than three hours
Convenient – perform a simple procedure without using ultracentrifugation over gradients
Complete – obtain cytoplasmic, membrane, soluble nuclear, chromatin-bound and cytoskeletal protein fractions from a single kit
Cultured Cells – developed and optimized for use with cultured mammalian cells; for tissue samples use the Subcellular Fractionation Kit for Tissues, Part No. 87790
Compatible – use with a variety of downstream applications including Western blotting, protein assays, electrophoretic mobility shift assays and reporter-gene and enzyme-activity assays
Small-scale – kit provides sufficient reagents to fractionate 50 cell pellets of 20μL each, equivalent to a total of 2g of cell paste or tissue
Includes:
Four extraction buffers, micrococcal nuclease enzyme, calcium chloride solution and protease inhibitor cocktail
Recommended for:
Extraction and enrichment of proteins from different cellular compartments; Separation and profiling of protein from the cytoplasm, nucleus, membrane and cytoskeleton from the same cell sample; Studying protein translocation
| Subcellular Protein Fractionation Kit | |
| Liquid | |
| Cell Lysis Buffer, Detergent Solution, Subcellular Fractionation | |
| 1 Kit |
| Upon receipt store kit at -20°C, or store individual components as indicated above. Kit is shipped with dry ice. | |
| Lysis Buffer | |
| 50 Samples |
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For Research Use Only. Not for use in diagnostic procedures.
