
PCR Equipment and Supplies

Thermo Scientific™ Phusion Flash High-Fidelity PCR Master Mix
Thermo Scientific Phusion Flash PCR Master Mix was developed to save valuable laboratory time.

Shipping Condition | Dry Ice |
---|---|
Polymerase | Phusion Flash II DNA Polymerase |
GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 25X |
Size (Final Product) | 20 kb or less |
Reaction Format | SuperMix or Master Mix |
Product Type | High-Fidelity PCR Master Mix |
Reaction Speed | Fast |
Color | Colorless |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Concentration | 2X |
For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR
Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.

Shipping Condition | Wet Ice |
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InhibitorToleranceSimple | Medium |
Concentration | 2X |
For Use With (Application) | Gene Expression |
PCR Method | qPCR |
Multiplex Capability | Single-plex |
Detection Method | SYBR |
Includes | dUTP, UNG/UDG |
Thermostability | 72 hr., Assembled Reaction |
Polymerase | Dual-Lock Taq DNA Polymerase |
Format | Tube |
Product Type | Real Time PCR SYBR Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 7 Flex, QuantStudio™ Absolute Q Digital PCR System, StepOne™, StepOnePlus™, ViiA™ 7 System |
Sample Type | DNA |
Reaction Speed | Fast or Standard |
Target Specificity | cDNA or gDNA only |
Product Line | PowerUP™, SYBR™ |
Applied Biosystems™ TaqPath™ 1-Step RT-qPCR Master Mix, CG
TaqPath 1-Step RT-qPCR Master Mix, CG, is designed for robust and reproducible one-step pathogen detection and gene expression workflows. The single-tube 4X formulation contains thermostable MMLV reverse transcriptase, dNTPs, UNG, ROX dye, and thermostable Fast DNA polymerase.

Reverse Transcriptase | M-MLV |
---|---|
Form | Liquid |
InhibitorToleranceSimple | High |
Concentration | 4X |
For Use With (Application) | Microbial Detection,DNA Quantitation,Presence/Absence |
PCR Method | 1-step RT-qPCR |
Multiplex Capability | Up to 3-plex |
Detection Method | Primer-probe |
Includes | dUTP, UNG/UDG |
Thermostability | Not Applicable |
Polymerase | Fast DNA Polymerase |
Sensitivity | Down to 10 copies |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System, 7500 Fast Dx System, QuantStudio™ Dx |
Sample Type | RNA, cDNA, DNA |
Reaction Speed | Fast or Standard |
Target Specificity | RNA only, Both RNA and DNA |
Hot Start | Built-In Hot Start |
Product Line | TaqPath™ |
Applied Biosystems™ SYBR™ Green Universal Master Mix
Everything needed for SYBR™ Green dye-based PCR amplification and detection in a convenient, single-tube format.

Polymerase | AmpliTaq Gold DNA Polymerase |
---|---|
GC-Rich PCR Performance | High |
Format | Tube |
Product Type | Real Time PCR SYBR Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
Reaction Speed | Standard |
Label or Dye | SYBR Green |
Concentration | 2X |
For Use With (Application) | Gene Expression |
PCR Method | qPCR |
Product Line | SYBR™ |
Detection Method | SYBR |
Applied Biosystems™ Region of Interest (ROI) and Background Plates, 96-well
Used to maintain the ViiA™ 7, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6, QuantStudio™ 7, and QuantStudio™ 12K Flex real-time PCR systems with 96-well (0.2 mL) blocks.

Thermo Scientific™ Phusion™ Plus PCR Master Mixes
Thermo Scientific Phusion Plus PCR Master Mix is a convenient, ready-to-use 2X mixture of Phusion Plus DNA Polymerase, buffer, magnesium chloride, and dNTPs.

Shipping Condition | Dry Ice |
---|---|
Polymerase | Phusion Plus DNA Polymerase |
GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | >100X |
Size (Final Product) | 20 kb or less |
Reaction Format | Ready to use master mix |
Reaction Speed | 15 to 30 sec./kb |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Concentration | 2X |
For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Thermo Scientific™ Phusion High-Fidelity PCR Master Mix with HF or GC Buffer
Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

Shipping Condition | Dry Ice |
---|---|
Polymerase | Phusion High-Fidelity DNA Polymerase |
Fidelity (vs. Taq) | 52X |
Size (Final Product) | 20 kb or less |
Reaction Format | SuperMix or Master Mix |
Product Type | High-Fidelity PCR Master Mix |
Reaction Speed | Fast |
Hot Start | No |
Overhang | Blunt |
Concentration | 2X |
For Use With (Application) | Standard PCR,High-fidelity PCR |
Applied Biosystems™ Protein Thermal Shift™ Dye Kit
This solution significantly reduces the time and cost associated with screening samples relative to currently available methods.

Applied Biosystems™ PowerTrack™ SYBR Green Master Mix for qPCR
PowerTrack SYBR Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows with built-in visual dye–based indicators for accurate reaction set up.

Shipping Condition | Dry Ice |
---|---|
Content And Storage | • PowerTrack SYBR Green Master Mix • 40X Yellow Sample Buffer Store at -20±5°C in the dark. |
InhibitorToleranceSimple | High |
Concentration | 2X |
For Use With (Application) | Gene Expression,Microbial Detection |
PCR Method | qPCR |
Multiplex Capability | Single-plex |
Detection Method | SYBR |
Includes | dUTP, UNG/UDG |
Thermostability | 8 hr., Assembled Reaction |
Polymerase | Taq DNA Polymerase |
Format | Tube |
Product Type | Real Time PCR SYBR Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Flex, QuantStudio 7 Pro, QuantStudio 12K Flex, QuantStudio™ Absolute Q Digital PCR System, Bio-Rad CFX Connect™, Bio-Rad CFX384 Touch™, Bio-Rad CFX96 Touch™, BioRad CFX96™, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, ViiA™ 7 System, StepOne™ System, MJ Chromo4, MJ Opticon, StepOnePlus™ System, Roche LightCycler 480, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000 |
Sample Type | DNA |
Reaction Speed | Fast or Standard |
Target Specificity | cDNA or gDNA only |
Product Line | Applied Biosystems™ |
Applied Biosystems™ StepOnePlus™ Real-Time PCR System Spectral Calibration Kit (96 well)
Easy-to-use kit establishes the pure dye spectra and multicomponent values for FAM™/SYBR™ Green I, VIC™/JOE™, and NED™/TAMRA™/ROX™ dyes on the StepOnePlus™ RT-PCR System.

Thermo Scientific™ DreamTaq DNA Polymerase (5 U/μL)
Thermo Scientific DreamTaq DNA Polymerase is an enhanced Taq DNA polymerase optimized for all standard PCR applications. It ensures higher sensitivity, longer PCR products, and higher yields compared to conventional Taq DNA polymerase.

Polymerase | DreamTaq DNA Polymerase |
---|---|
GC-Rich PCR Performance | Low |
Fidelity (vs. Taq) | 1X |
Reaction Speed | Standard |
Hot Start | No |
Overhang | 3'-A |
Concentration | 5 U/μL |
Invitrogen™ Second Strand cDNA Synthesis Kit
Invitrogen™ Second Strand cDNA Synthesis Kit is design to produce double-stranded cDNA using first strand reaction without the need for intermediate organic extraction or ethanol precipitation steps. This convenient single-tube format speeds up the synthesis procedure and maximizes cDNA recovery. The kit contains premixed components to reduce the number of pipetting steps necessary to complete the procedure.

Reaction Format | Separate Components |
---|---|
Final Product Type | Double Stranded cDNA |
Product Type | cDNA Synthesis Kit |
For Use With (Application) | Cloning |
Invitrogen™ Platinum™ Taq DNA Polymerase
Invitrogen Platinum Taq DNA Polymerase is a convenient and reliable 'hot start' thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase.

Polymerase | Platinum Taq DNA Polymerase |
---|---|
GC-Rich PCR Performance | Low |
Fidelity (vs. Taq) | 1X |
Format | Tube |
Size (Final Product) | 5 kb or less |
Reaction Format | Separate Components |
Product Type | DNA Polymerase |
Hot Start | Built-In Hot Start |
Overhang | 3'-A |
Starting Material | DNA |
For Use With (Application) | Hot-start PCR |
Exonuclease Activity | 5' - 3' |
Thermo Scientific™ DreamTaq PCR Master Mixes (2X)
Thermo Scientific DreamTaq PCR Master Mix (2X) is a ready-to-use solution containing DreamTaq DNA Polymerase, optimized DreamTaq buffer, MgCl2, and dNTPs.

Shipping Condition | Dry Ice |
---|---|
Polymerase | DreamTaq DNA Polymerase |
GC-Rich PCR Performance | Low |
Fidelity (vs. Taq) | 1X |
Reaction Format | SuperMix or Master Mix |
Reaction Speed | Standard |
Hot Start | No |
Overhang | 3'-A |
For Use With (Application) | Standard PCR |
First Strand cDNA Synthesis Kit
Thermo Scientific™ Fermentas First Strand cDNA Synthesis Kit is a complete system for the efficient synthesis of first strand cDNA from RNA templates.
