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Applied Biosystems™ Power SYBR™ Green RNA-to-CT™ 1-Step Kit
The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets.
Brand: Applied Biosystems™ 4389986
489.50 EUR valid until 2024-12-20
Use promo code "21651" to get your promotional price.
This item is not returnable.
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Description
The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets.
- Get sensitivity and specificity in an easy-to-use one-step qRT-PCR reaction.
- Formulated to ensure maximum sensitivity and reliability
- Reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets
Includes:
- AmpliTaq™ Gold DNA Polymerase UP for hot start qPCR, minimizing non-specific product formation
- ArrayScript™ UP Reverse Transcriptase, an engineered RT that produces high yields of full-length cDNA
- An additive that reduces primer dimer formation, reducing non-specific products that can cause false positive signals
- RNase inhibitor, to prevent degradation of RNA templates
- A proprietary ROX™ dye that serves as a passive internal reference to normalize non-PCR-related fluorescence fluctuations for superb precision on Applied Biosystems™ real-time PCR instruments
Order Info
Shipping Conditions: Wet Ice
Specifications
Sufficient for 200 reactions at 50 μL reaction volume and includes: • 1 x 5 mL tube of 2X master mix containing SYBR™Green 1 Dye, AmpliTaq Gold™ DNA Polymerase UP, dNTPs, Passive Reference 1,and optimized buffer components. Store at 2-8°C after opening. • 1 x 80 μL tube of 125X RT enzyme mix containing ArrayScript™ UP Reverse Transcriptase, RNase Inhibitor. Store at -15 to -30°C. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA). |
|
1-step RT-qPCR | |
Standard | |
SYBR Green | |
RNA-to-CT™, SYBR™ | |
UP (Ultra Pure) | |
Standard | |
200 Reactions | |
200 Reactions |
SYBR | |
AmpliTaq Gold DNA Polymerase | |
7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System | |
ROX (Pre-mixed) | |
One-Step qRT-PCR Kit | |
ArrayScript™ UP | |
Wet Ice | |
Gene Expression | |
RNA |
For Research Use Only. Not for use in diagnostic procedures.