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Thermo Scientific™ Tango™ EDG1-bla U2OS Cells

Brand: Thermo Scientific™ K1520

Product Code. 10489228

13050.00€ / Each

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Estimated Shipment: 02-04-2025
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Description

The Tango™ EDG1-bla U2OS cells contain the human Endothelial Differentiation, Sphingolipid G-protein-coupled Receptor, 1 (EDG1) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.

The Tango™ EDG1-bla U2OS cells cells have been functionally validated for Z' factor and EC50 concentrations of established ligands. In addition, Tango™ EDG1-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary for each product.

Specifications

Accession Number	NM_001400.2
Cell State	Dividing Cells
Cytoplasmic Tail	Native Tail
For Use With (Application)	GPCRs
Includes	EDG1-bla U2OS cells, EDG1-bla U2OS Assay Protocol, Certificate of Analysis
Product Line	Tango™
Reporter Gene	BLA (Beta-Lactamase)
Type	U2OS Cell
System Type	Tango™

Assay Entry	Cell-based beta-arrestin recruitment
Content And Storage	LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol. Includes: • Tango™ EDG1-bla U2OS cells • Tango™ EDG1-bla U2OS Assay Protocol • Certificate of Analysis The Tango™ EDG1-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Detection Method	Fluorescence
Gene Symbol	EDG1
Population Status	Single Cell Clone Origin
Quantity	2 x 10⁶ Cells
Target Entry	EDG1
Cell Line	U2OS
Druggable Target	GPCR

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Thermo Scientific™ Tango™ EDG1-bla U2OS Cells

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