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Thermo Scientific™ Tango™ HTR1A-bla U2OS Cells

Brand: Thermo Scientific™ K1857

Product Code. 10175694

13050.00€ / Each

Quantity

Estimated Shipment: 02-04-2025
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Description

Tango™ HTR1A-bla U2OS cells contain the human Serotonin Type 1a receptor (HTR1A) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase reporter gene under the control of a UAS response element. Tango™ HTR1A-bla U2OS DA cells have been functionally validated for Z' factor and EC50 concentrations of 5-CT (Figure 1). In addition, Tango™ HTR1A-bla U2OS cells have been tested for assay performance under variable conditions.

Specifications

Accession Number	NM_000524
Cell State	Dividing Cells
Cytoplasmic Tail	Tail from AVPR2 GPCR
For Use With (Application)	FRET
Human Protein Class	G-Protein Coupled Receptor (GPCR)
Population Status	Single Cell Clone Origin
Quantity	8 x 10⁶ Cells
Target Entry	5HT1A, HTR1A
Cell Line	U2OS
Druggable Target	GPCR

Assay Entry	Cell-based beta-arrestin recruitment
Content And Storage	LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol. Includes: • Tango™ HTR1A-UAS-bla CHO-K1 cells • Tango™ HTR1A-UAS-bla CHO-K1 Assay Protocol • Certificate of Analysis The Tango™ HTR1A-UAS-bla CHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Detection Method	Fluorescence
Gene Symbol	HTR1A
Includes	HTR1A-UAS-bla CHO-K1 cells, HTR1A-UAS-bla CHO-K1 Assay Protocol, Certificate of Analysis
Product Line	Tango™
Readout	End Point
Type	Dividing Cells
System Type	Tango™

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Thermo Scientific™ Tango™ HTR1A-bla U2OS Cells

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