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Cytiva HisTrap™ excel Prepacked Columns
For capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants. Cytiva Lifescience™ HisTrap™ excel Prepacked Columns are designed for fast and convenient purifications.
70.00€ - 913.00€
Specifications
| Description | HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC). |
|---|---|
| Material | Polypropylene |
| pH Range | 2 to 12 |
| Support Type | 6% Highly Cross-linked Agarose |
| Content And Storage | 4°C to 30°C |
| Product Code | Brand | Diameter (Metric) Inner | Quantity | Flow Rate | Price | Quantity & Availability | |||
|---|---|---|---|---|---|---|---|---|---|
| Product Code | Brand | Diameter (Metric) Inner | Quantity | Flow Rate | Price | Quantity & Availability | |||
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12632966
|
Cytiva
17-3712-05 |
7 mm | 5 x 1 mL | 1 to 4 mL/min. |
270.00€
5mL |
In Stock
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12683466
|
Cytiva
17-3712-06 |
16 mm | 5 x 5 mL | 5 to 20 mL/min. |
913.00€
Pack of 5 |
In Stock
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|
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|
15187946
|
Cytiva
29048586 |
7 mm | 1 mL | 1 to 4 mL/min. |
70.00€
1mL |
In Stock
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Description
The design of the columns in combination with the specific properties of the medium enables fast and convenient purifications.
- Ready-to use prepacked with Ni Sepharose excel
- Special type of filter in top and bottom of the columns to load large volumes of cell-free, un clarified samples directly on the columns helps prevent degradation and loss of sensitive target proteins
- Made of biocompatible polypropylene and cannot be opened or refilled
- Delivered with stopper on inlet and snap-off end on outlet
- Operated either with peristaltic pump or with chromatography systems such as KTA systems
- Binding Capacity/Column: At least 10 mg histidine-tagged protein/ml sedimented medium
- Average particle size: 90μm
- Matrix: Highly cross-linked agarose, 6%
- pH Stability working range: 2 to 12
- pH Stability cleaning range: 2 to 14
- Flow velocity: 150 cm/h - 600 cm/h4
- Column I.D.: 7 mm
HisTrap excel 1mL column
- Bed Dimensions: 7 × 25mm
- Flow rate: 1 to 4mL/min.
- Bed Dimensions: 16 × 25mm
- Flow rate: 5 to 20mL/min.
Specifications
| HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC). | |
| 2 to 12 | |
| 4°C to 30°C | |
| Pre-packed Column |
| Polypropylene | |
| 6% Highly Cross-linked Agarose | |
| 5 bar (0.5 MPa, 70 psi) |
Safety and Handling
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Warning
Hazard Category
- Flammable liquid Category 3
Hazard Statement
- H226-Flammable liquid and vapour.
Precautionary Statement
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P235-Keep cool.
- P501-Dispose of contents/container to .
Supplemental information
- MIXTURE LIST-Contains : ethanol