missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Protein Purification
  5. Protein Purification Reagents and Kits
  6. Cytiva HisTrap™ excel Prepacked Columns

Cytiva HisTrap™ excel Prepacked Columns

Product Code. 15187946 Shop All Cytiva Products
Click to view available options
Quantity:
1 mL
5 x 1 mL
5 x 5 mL
Diameter (Metric) Inner:
7 mm
16 mm
Flow Rate:
1 to 4 mL/min.
5 to 20 mL/min.
missing translation for 'unitSize'
1mL
5mL
Pack of 5
Les retours ne sont pas autorisés pour ce produit. Afficher la politique du retour.

Code produit. 15187946

missing translation for 'mfr': Cytiva 29048586

Il en reste null
Ajouter au panier
Ajouter au panier
Les retours ne sont pas autorisés pour ce produit. Afficher la politique du retour.

Description

For capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants. Cytiva Lifescience™ HisTrap™ excel Prepacked Columns are designed for fast and convenient purifications.

The design of the columns in combination with the specific properties of the medium enables fast and convenient purifications.

  • Ready-to use prepacked with Ni Sepharose excel
  • Special type of filter in top and bottom of the columns to load large volumes of cell-free, un clarified samples directly on the columns helps prevent degradation and loss of sensitive target proteins
  • Made of biocompatible polypropylene and cannot be opened or refilled
  • Delivered with stopper on inlet and snap-off end on outlet
  • Operated either with peristaltic pump or with chromatography systems such as KTA systems
  • Binding Capacity/Column: At least 10 mg histidine-tagged protein/ml sedimented medium
Media
  • Average particle size: 90μm
  • Matrix: Highly cross-linked agarose, 6%
  • pH Stability working range: 2 to 12
  • pH Stability cleaning range: 2 to 14
  • Flow velocity: 150 cm/h - 600 cm/h4
Column:
  • Column I.D.: 7 mm

HisTrap excel 1mL column

  • Bed Dimensions: 7 × 25mm
  • Flow rate: 1 to 4mL/min.
HisTrap excel 5mL column
  • Bed Dimensions: 16 × 25mm
  • Flow rate: 5 to 20mL/min.

TRUSTED_SUSTAINABILITY

Spécification

Bed Volume 1 mL
Diameter (English) Inner 0.2 in.
Diameter (Metric) Inner 7 mm
Description HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).
Flow Rate 1 to 4 mL/min.
Material Polypropylene
pH Range 2 to 12
Support Type 6% Highly Cross-linked Agarose
Content And Storage 4°C to 30°C
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
Quantity 1 mL
Type Pre-packed Column
Afficher plus Afficher moins

Sécurité et traitement

compliance-icons compliance-icons
Mention d'avertissement
  • Warning
 Catégorie de danger
  • LONG-TERM AQUATIC HAZARD Chronic 2
  • Flammable liquid Category 3
 Mention de danger
  • H226-Flammable liquid and vapour.
  • H411-Toxic to aquatic life with long lasting effects.
 Conseils de prudence
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Informations supplémentaires
  • MIXTURE LIST-Contains : ethanol, nickel
  • UNKNOWN ACUTE-16.5 per cent of the mixture consists of component(s) of unknown acute toxicity.
Correction du contenu d'un produit

Veuillez fournir vos retours sur le contenu du produit en remplissant le formulaire ci-dessous.

Nom du produit

En cliquant sur Soumettre, vous reconnaissez que vous pouvez être contacté par Fisher Scientific au sujet des informations que vous avez fournies dans ce formulaire. Nous ne partagerons pas vos informations à d'autres fins. Toutes les informations de contact fournies seront également conservées conformément à notre politique de confidentialité. Politique de confidentialité.

Merci de nous aider à améliorer notre site web. Votre commentaire a été soumis