Test
One Shot TOP10 Chemically Competent E. coli are ideal for high-efficiency cloning and plasmid DNA propagation and are provided at a transformation efficiency of 1 x 109 cfu/μg plasmid DNA.
Shipping Condition | Dry Ice |
---|---|
Blue/White Screening | Yes (lacZΔM15) |
Cloning Methylated DNA | Yes (mcrA) |
Transformation Efficiency Level | High Efficiency (>1 x 109 cfu/μg) |
High-throughput Compatibility | Low |
Improves Plasmid Quality | Yes (endA1) |
Preparing Unmethylated DNA | No |
Plasmid | High Copy Plasmid |
Reduces Recombination | Yes (recA1) |
T1 Phage - Resistant (tonA) | No |
Antibiotic Resistance Bacterial | Yes (Streptomycin) |
Format | Tube |
Product Type | Chemically Competent Cells |
Species | E. coli (K12) |
Product Line | One Shot™ |
Contains F' Episome | No |
High efficiency, chemically competent E. coli cells ideal for construction of gene banks or generation of cDNA libraries using plasmid-derived vectors
MAX Efficiency™ DH5α Competent Cells are a well-known, versatile strain that is used in many everyday cloning applications. Prepared by a proprietary modification of the Hanahan method, these cells are suitable for high efficiency transformations in applications where low amount of DNA is used or to construct libraries using plasmid-derived vectors.
Chemically competent E. coli cells recommended for routine subcloning into plasmid vectors. Subcloning efficiency cells are not suitable for the generation of cDNA libraries.
Suitable for the expression of non-toxic heterologous genes. The strain contains the lambda DE3 prophage that carries the gene for T7 RNA polymerase under control of a lacUV5 promoter, allowing expression of the T7 RNA polymerase to be induced with IPTG.
Subcloning Efficiency DH5α Competent Cells are a versatile, chemically competent strain for cloning that provides a transformation efficiency of >1 x 106 cfu/μg plasmid DNA.
One Shot Stbl3 Chemically Competent E. coli are designed for cloning direct repeats found in lentiviral expression vectors as these cells reduce the frequency of homologous recombination of direct repeats found in lentiviral vectors (ViraPower Lentiviral Expression kits) and other retroviral vectors (instability-prone HIV-based plasmids).
Library Efficiency DH5α Competent Cells are a versatile strain of chemically competent cells that are used in many everyday cloning applications.
One Shot BL21 Star (DE3) Chemically Competent E. coli are designed for applications that require high-level expression of non-toxic recombinant proteins from low copy number, T7-promoter-based expression systems such as Champion pET vectors.
One Shot ccdB Survival 2 T1R Competent Cells are suitable for the propagation of plasmids containing the ccdB gene and are designed for use with the Gateway Vector Conversion System and for propagating Gateway destination, donor, and supercoiled entry vectors.
Capable of supporting site-specific recombination between pFastBac™ and bMON14272
High efficiency, chemically competent E. coli cells. The DH10B strain is suitable for cloning DNA containing methylcytosine, 5-hydroxymethylcytosine, and methyladenine, allowing both prokaryotic and eukaryotic genomic DNA to be cloned efficiently.
MAX Efficiency DH5αF'IQ Competent Cells are high transformation efficiency chemically competent cells prepared by a patented modification of the procedure of Hanahan. This strain is a derivative of the DH5α strain, sharing the same benefits.
One Shot BL21(DE3)pLysS Chemically Competent E. coli cells are ideal for use with bacteriophage T7 promoter-based expression systems such as pRSET and pET.
Shipping Condition | Dry Ice |
---|---|
Blue/White Screening | No |
Cloning Methylated DNA | No |
Transformation Efficiency Level | Medium Efficiency (1 x 108 to 1 x 109 cfu/μg) |
ToxicProteins | No |
High-throughput Compatibility | Low |
Improves Plasmid Quality | No |
ImprovesProteinStability | Yes (lon, ompT) |
Promoter | T7 |
Preparing Unmethylated DNA | Yes (dcm) |
Reduces Recombination | No |
ImprovesRNAStability | Yes (pLysS) |
T1 Phage - Resistant (tonA) | No |
Antibiotic Resistance Bacterial | Yes (Chloramphenicol) |
Format | Tube |
Product Type | Chemically Competent Cells |
Species | E. coli (B) |
Product Line | One Shot™ |
Contains F' Episome | No |
One Shot BL21(DE3) Chemically Competent E. coli cells are ideal for use with bacteriophage T7 promoter-based expression systems such as pRSET and pET.