missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Competent Cells
  5. 16575681

Thermo Scientific™ DH10B Competent Cells; High Efficiency

High efficiency, chemically competent E. coli cells. The DH10B strain is suitable for cloning DNA containing methylcytosine, 5-hydroxymethylcytosine, and methyladenine, allowing both prokaryotic and eukaryotic genomic DNA to be cloned efficiently.

Brand:  Thermo Scientific™ EC0113

115.34 EUR valid until 2024-12-31
Use promo code "21651" to get your promotional price.

Product Code. 16575681

  • 158.00€ / 20 reactions

Please to purchase this item. Need a web account? Register with us today!

Edge
Explore more special offers
This item is not returnable. View return policy

Description

Description

  • Suitable for cloning DNA containing methylcytosine, 5-hydroxymethylcytosine, and methyladenine, allowing both prokaryotic and eukaryotic genomic DNA to be cloned efficiently
  • These cells are ideal for the construction of gene banks or for the generation of cDNA libraries using plasmid-derived vectors
  • The φ80dlacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors to allow blue/white colony screening on bacterial agar plates containing Bluo-Gal or X-Gal
  • High transformation efficiency: >1x109 cfu/μg pUC19 DNA
  • Suitable for routine and high-throughput cloning applications
  • Allows for blue/white colony screening
  • Convenient two reactions per vial packaging
  • S.O.C. Outgrowth medium included
  • Suitable for variety of applications requiring high transformation efficiency:
    • Efficient DNA cloning both of prokaryotic and eukaryotic origin
    • Cloning of large plasmids and BACs
    • Ideal for generation of cDNA libraries using plasmid-derived vectors
    • Site directed mutagenesis
  • Genotype: F– mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ (ara-leu)7697 galU galK λ– rpsL(StrR) nupG

Note: Optimized for use with the CloneJET PCR Cloning Kit (Cat. Nos. K1231, K1232) and Phusion Site-Directed Mutagenesis Kit (Cat. No. F541), and available as combo versions (Cat. Nos. K123120, K123240, F542)

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Chemically Competent
Dry Ice (-80°C)
E. coli
Cloning
Lacks F' Episome
DH10B
Tube
20 Reactions
Product Suggestions

Product Suggestions

Videos
SDS
Documents

Documents

Certificates
Promotions

Promotions

Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Thank You! Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.