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Cytiva Protein A and Protein G Mag Sepharose™
Simplify enrichment of target proteins by immunoprecipitation or pull-down applications
Brand: Cytiva 28-9513-78
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Description
Protein A and Protein G Mag Sepharose are magnetic beads designed to simplify enrichment of target proteins by immunoprecipitation or pull-down applications. The magnetic beads are based on Sepharose with native protein A or native protein G as ligand. Protein A and Protein G have high affinities for the Fc region of IgG antibodies and offer differing affinity characteristics across a broad range of mammalian IgG species.
There are two protocols available for protein enrichment: the cross-link protocol and the classic protocol. The protocols are simple with elution conditions optimized for for downstream applications such as electrophoresis and MS.
Protein A and Protein G Mag Sepharose combine well-proven enrichment methods with the magnetic beads platform, which has ideal properties for small-scale sample preparation. Together with MagRack 6, a separation tool for six microcentrifuge tubes, up to six samples can be processed in parallel.
- Visible and dense Sepharose based magnetic beads with protein A or protein G ligand, easy to spot and collect bound target protein
- Non-adherent beads eliminate smearing effects and aggregate formation. Can be used without detergents
- Simple capture of target protein in small or large sample volumes (low microliter to high milliliter scale)
- Optimized capacity for enrichment/immunoprecipitation of target proteins - only low amounts of antibody needed
Matrix: Paramagnetic, spherical, highly cross-linked agarose particles
Ligand: Native protein A; Native protein G
Binding capacity Protein A: 8-17mg human IgG/mL gel
Binding capacity Protein G: 13-22mg human IgG/mL gel
Particle size: 37-100μm
Working temperature: Room temperature
Storage solution: 20% ethanol
Storage temperature: 4°C to 8°C
Specifications
| Protein A Mag Sepharose | |
| Paramagnetic spherical, highly cross linked agarose particles | |
| 4 x 500 μL | |
| Designed for covalent coupling of any Protein or biomolecule containing a free Amine group and is used for various types of pull down experiments, for biomarker research |
| 8 to 17mg Human IgG/mL | |
| 37 to 100 μm | |
| 4°C to 8°C | |
| Native Protein A |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Protein A Mag Sepharose
Signal Word
- Warning
Hazard Category
- Flammable liquid Category 3
Hazard Statement
- H226-Flammable liquid and vapour.
Precautionary Statement
- P240-Ground/bond container and receiving equipment.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : ethanol
- UNKNOWN ACUTE-66.5 per cent of the mixture consists of component(s) of unknown acute toxicity.
- UNKNOWN AQUATIC-Contains 50% of components with unknown hazards to the aquatic environment.
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