Learn More
Thermo Scientific™ Pierce™ ECL Plus Western Blotting Substrate
Acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.
142.00€ - 780.00€
Specifications
Product Type | Substrate |
---|---|
Detection Method | Chemiluminescence, Fluorescence |
Form | Liquid |
Membrane Compatibility | Nitrocellulose, PVDF |
Product Line | Pierce™ |
Product Code | Brand | Quantity | Sufficient For | Price | Quantity & Availability | ||||
---|---|---|---|---|---|---|---|---|---|
Product Code | Brand | Quantity | Sufficient For | Price | Quantity & Availability | ||||
11547910
|
Thermo Scientific™
32134 |
25 mL Kit | 250 sq. cm of membrane |
142.00€
25mL |
Please sign in to purchase this item. Need a web account? Register with us today! | ||||
11527271
|
Thermo Scientific™
32132X3 |
300 mL Kit | 3000 sq. cm of membrane |
780.00€
Pack of 3 |
Please sign in to purchase this item. Need a web account? Register with us today! | ||||
11517271
|
Thermo Scientific™
32132 |
100 mL Kit | 10 mini-gel blots; 1000cm2 of membrane |
326.00€
100mL |
In Stock
Log in to see stock. |
||||
Description
Thermo Scientific™ Pierce ECL Plus Substrate is an acridan-based chemiluminescent and chemifluorescent substrate to detect peroxidase activity from HRP-conjugated probes by Western blotting using X-ray film, CCD or laser-based imagers.
Pierce ECL Plus Substrate is a chemiluminescent and chemifluorescent substrate to detect peroxidase activity from HRP-conjugated antibodies and probes by Western blotting using X-ray film, CCD camera documentation systems or laser-based imagers. Pierce ECL Plus Substrate generates acridinium esters when it reacts with HRP. As these ester intermediates react with peroxide, they produce a strong and sustained chemiluminescent signal that can be captured by X-ray film CCD imaging. The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers. Pierce ECL Plus Substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations. Its broad dynamic range, high sensitivity and long-lasting signal make Pierce ECL Plus Substrate an excellent choice for Western blotting.Highlights:
Easy to use — can be substituted for the discontinued GE ECL Plus Substrate without any re-optimization
Higher sensitivity — detect targets down to the low-picogram level
Longer signal duration — sustained light output for as long as five hours
More imaging options — X-ray, CCD or laser-based imagers
More affordable — high quality and performance at a lower price than other suppliers« similar substrates
Specifications
Substrate | |
Liquid | |
Pierce™ |
Chemiluminescence, Fluorescence | |
Nitrocellulose, PVDF | |
Low picogram to mid-femtogram |
Safety and Handling
- Pierce ECL Plus Western Blotting Substrate
Signal Word
- Danger
Hazard Category
- Carcinogenicity Category 2
- Serious eye damage/eye irritation Category 2
- Flammable liquid Category 2
- Specific target organ toxicity Category 3
Hazard Statement
- H225-Highly flammable liquid and vapour.
- H319-Causes serious eye irritation.
- H335-May cause respiratory irritation.
- H351-Suspected of causing cancer.
Precautionary Statement
- P201-Obtain special instructions before use.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P403+P235-Store in a well-ventilated place. Keep cool.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : Ethanol
For Research Use Only. Not for use in diagnostic procedures.