Western Blot Products
Western Blot Products
Kits, buffers, blocking agents, reagents, membranes and substrates for use in conducting western blotting and ELISA immunoassays; includes accessories, modules, and replacement parts and solutions. Steps include: sample preparation, gel electrophoresis, membrane transfer, antibody probe, detection, imaging, and analysis.
The western blot (WB) is a common protein detection and analysis method. A protein sample is denatured to prepare it for gel electrophoresis. Following separation by electrophoresis, and then transferred (blotted) from the gel to a membrane for specific visualization. The blot is covered with a buffer that contains an antibody that will bind to specific parts of the protein being studied. A secondary antibody (an antibody to the primary antibody) is then added (to bind to the primary antibody). The secondary antibody is then visualized by staining, immunofluorescence, radioactivity, or other methods.
Search our extensive portfolio of primary antibodies and secondary antibodies specifically designed for western blotting.
Cytiva Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls
Use this versatile nitrocellulose membrane for excellent binding affinity for small proteins and peptides, as well as nucleic acids. Cytiva Amersham™ Protran™ NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.
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Thermo Scientific™ PVDF Transfer Membrane, 0.2μm, 26.5 cm x 3.75 m
PVDF Transfer Membrane, 0.2 μm, 26.5 cm x 3.75 m
Cytiva Amersham™ ECL Select™ Western Blotting Detection Reagent
Use as a high sensitivity reagent for chemiluminescent Western blotting detection. Cytiva Life Sciences™ Amersham™ ECL Select™ Western Blotting Detection Reagent provides highly intense signal output, for detection of medium to very low protein levels.
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Cytiva Amersham™ ECL™ Prime Western Blotting Detection Reagent
Enables the use of highly diluted primary and secondary antibodies without reducing sensitivity. Cytiva Lifescience™ Amersham™ ECL™ Prime Western Blotting Detection Reagent produces high signal intensity and sensitivity.
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Cytiva Amersham™ Hybond™ P 0.45μm PVDF Membrane
Use for chemiluminescent and fluorescent detection methods for proteins of > Mr 20 000. Cytiva Life Sciences™ Amersham™ Hybond™ P 0.45μm PVDF Membrane is a 0.45 μm pore size hydrophobic membrane, with high physical strength, highly suitable for stripping and reprobing.
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Thermo Scientific™ CL-XPosure™ Film
Thermo Scientific CL-XPosure Film is an economically priced clear-blue X-ray film especially for detection and documentation of ECL-based western blots and other chemiluminescent protein or nucleic acid assay methods.
Cytiva Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls
Use for the best results with fluorescence Western blotting detection applications. Cytiva Life Sciences™ Amersham™ Protran™ Premium NC Nitrocellulose Membranes: Rolls offer excellent sensitivity, resolution, and low background.
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Thermo Scientific™ Blocker™ BSA
Blocker BSA is a purified 10% solution of bovine serum albumin for blocking in western blotting, ELISA, IHC, and nucleic acid detection methods.
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Merck Immobilon™ Western Chemiluminescent HRP Substrate (ECL)
Provides high sensitivity over a broad detection range at an economical price
Cytiva Amersham™ Hybond™ PVDF Membranes: Rolls
Ideal for Western blotting applications where stripping and reprobing are necessary. Cytiva Life Sciences™ Amersham™ Hybond™ PVDF Membranes: Rolls are hydrophobic PVDF membranes.
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Cytiva Whatman™ Grade GB005 Blotting Paper
Recommended for applications where fewer layers of blotting paper must still ensure a high capacity. Whatman™ Grade GB005 Blotting Paper ensures the wicking capability and uniformity of capillary action that are important in obtaining clean and even transfers during blotting.
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Cytiva Amersham™ ECL™ Western Blotting Detection Reagents
Use for all routine Western blotting. Cytiva Life Sciences™ Amersham™ ECL™ Western Blotting Detection Reagents are the world's first commercially available chemiluminescent detection reagents and still one of the most widely used.
Thermo Scientific™ SuperSignal™ West Femto Maximum Sensitivity Substrate
Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.
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Thermo Scientific™ Restore™ PLUS Western Blot Stripping Buffer
Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.
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Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate
Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram to high femtogram-level protein detection by western blot analysis.
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