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Thermo Scientific™ Klenow Fragment (10 U/μL)

Klenow Fragment is the Large Fragment of DNA Polymerase exhibiting 5'→3' polymerase, 3'→5' exonuclease (proofreading) activities, but lacking 5'→3' exonuclease activity.

Brand:  Thermo Scientific™ EP0052

239.34 EUR valid until 2025-03-21
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Product Code. 10795071

  • 359.00€ / 1500 units

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Description

Description

Thermo Scientific Klenow Fragment is the large fragment of DNA polymerase I. It exhibits 5'→3' polymerase activity and 3'→5' exonuclease (proofreading) activity, but lacks 5'→3' exonuclease activity of DNA polymerase I.

Highlights

  • Incorporates modified nucleotides (e.g., Cy3-, Cy5-, aminoallyl-, biotin-, digoxigenin- and fluorescently-labeled nucleotides)
  • Active in restriction enzyme, PCR, RT, and T4 DNA Ligase buffers

Applications

  • DNA blunting by fill-in 3'-overhangs
  • Random-primed DNA labeling
  • Labeling by fill-in 5'-overhangs of dsDNA
  • DNA sequencing by the Sanger method
  • Site-specific mutagenesis of DNA with synthetic oligonucleotides
  • Second strand synthesis of cDNA
TRUSTED_SUSTAINABILITY
Specifications

Specifications

10 U/μL
DNA Polymerase I
1,500 units
Klenow Fragment
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Lot Number Certificate Type Date Catalog Number
Lot Number2807403 Certificate TypeCertificate of Analysis Date29/01/2025 Catalog NumberEP0052
Lot Number2789455 Certificate TypeCertificate of Analysis Date29/01/2025 Catalog NumberEP0052
Lot Number2837210 Certificate TypeCertificate of Analysis Date29/01/2025 Catalog NumberEP0052
Lot Number2837273 Certificate TypeCertificate of Analysis Date29/01/2025 Catalog NumberEP0052
Lot Number2840687 Certificate TypeCertificate of Analysis Date29/01/2025 Catalog NumberEP0052
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Thermo Scientific™ Klenow Fragment (10 U/μL) > 10μ/μL, 1500U

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For Research Use Only. Not for use in diagnostic procedures.