MAX Efficiency DH10B Competent Cells are highly efficient E. coli suitable for a variety of molecular biology applications. This strain is one of the most commonly used strains for everyday applications due to key strain features such as high transformation efficiency.
Brand: Invitrogen™ 18297010
151.34 EUR valid until 2025-03-29
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MAX Efficiency DH10B Competent Cells are highly efficient E. coli suitable for a variety of molecular biology applications. This strain is one of the most commonly used strains for everyday applications due to key strain features such as high transformation efficiency, making them ideal for generating plasmid libraries, an ability to take up and maintain large plasmids (seen with plasmids of 150 kb in size), and a lack of methylation-dependent restriction systems.
Benefits of MAX Efficiency DH10B Competent Cells
• Ability to maintain large DNA plasmids
• Resistant to the effects of ligase and ligase buffer and tolerant of small amounts of undiluted ligation reactions
• Suitable for the construction of cDNA or genomic DNA libraries using plasmid-derived vectors
Universal strain for everyday applications
The presence of the mcrA genotypic marker and the deletion of mcrBC and mrr makes this strain suitable for cloning DNA that contains methylcytosine and methyladenine. Therefore, genomic DNA, both prokaryotic and eukaryotic, can be cloned efficiently in DH10B.
Versatile DH10B genotype features
• lacZ∆M15 for blue/white screening of recombinant clones
• Elimination of mcrA, mcrBC, mrr, and hsdRMS restriction systems to allow construction of more representative genomic libraries
• endA1 mutation to increase plasmid yield and quantity
Genotype
F–mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara-leu)7697 galU galK λ–rpsL(StrR) nupG
Find the strain and format that fits your need
• DH10B Cells are available in both electrocompetent and chemically competent formats.
• MegaX DH10B T1R Electrocomp Cells (Cat. No.C640003) have the highest transformation efficiency (>3 x 1010 cfu/μg plasmid DNA).
• Multishot formats are available for high throughput applications.
Shipping Condition: Dry Ice
| Chemically Competent Cells | |
| Yes (endA1) | |
| High Efficiency (>1 x 109 cfu/μg) | |
| Yes (Streptomycin) | |
| Yes (lacZΔM15) | |
| No | |
| Dry Ice | |
| E. coli (K12) | |
| MAX Efficiency™ |
| No | |
| Yes (mcrA) | |
| • MAX Efficiency DH10B Competent Cells (5 x 200 μL) Store Competent Cells at –70°C • pUC19 DNA (100 μL at 0.01 μg/mL) Store pUC19 DNA at –20°C. • S.O.C. Medium (2 x 6 mL) Store S.O.C. Medium at 4°C or room temperature. |
|
| Not suitable for cloning unstable DNA | |
| Low | |
| Yes (recA1) | |
| No | |
| Tube | |
| 5 x 200 μL |
A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.
| Lot Number | Certificate Type | Date | Catalog Number |
|---|---|---|---|
| 2455206 | Certificate of Analysis | 29/01/2025 | 18297010 |
| 2524766A | Certificate of Analysis | 29/01/2025 | 18297010 |
| 2602322 | Certificate of Analysis | 29/01/2025 | 18297010 |
| 2822922A | Certificate of Analysis | 28/01/2025 | 18297010 |
| 2822949 | Certificate of Analysis | 25/01/2025 | 18297010 |
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