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Cytiva HisTrap™ HP Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Brand:  Cytiva 29051021


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Regulatory: This product contains a substance listed in Annex XIV to REACH Regulation (EC) No 1907/2006, all customers must complete a declaration before delivery.

Product Code. 15167956

  • 65.00€ / 1mL
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Description

Description

  • Prepacked with Ni Sepharose™ High Performance medium
  • Negligible nickel ion leakage
  • Pressure Max: 3 bar
  • Column material: polypropylene
  • 1 and 5mL columns
  • Compatible with a wide range of reducing agents, detergents, denaturants, and other additives
  • Manual operation with syringe, pump or chromatography system

Media
  • Matrix: 6% cross-linked agarose
  • Average particle size: 34μm
  • pH stability working range: 3 to 12

1mL columns

  • Bed Dimensions: 7 x 25mm
  • Flow velocity: 4mL/min.

5mL columns
  • Bed Dimensions: 16 x 25mm
  • Flow velocity: 5mL/min.

TRUSTED_SUSTAINABILITY
Specifications

Specifications

1 mL
7 mm
4 mL/min. (1 mL), 5 mL/min. (5 mL)
3 to 12
4°C to 30°C
1 mL
0.2 in.
HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery.
Polypropylene
6% Highly Cross-linked Agarose
5 bar (0.5 MPa, 70 psi)
Pre-packed Column
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Safety and Handling

Safety and Handling

Signal Word
  • Warning

Hazard Category
  • LONG-TERM AQUATIC HAZARD Chronic 3
  • Flammable liquid Category 3
  • Flammable liquid Category 3

Hazard Statement
  • H226-Flammable liquid and vapour.
  • H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information
  • MIXTURE LIST-Contains : ethanol, nickel
  • UNKNOWN ACUTE-16.5 per cent of the mixture consists of component(s) of unknown acute toxicity.

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