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Thermo Scientific™ GeneJET RNA Purification Kit
Thermo Scientific GeneJET RNA Purification Kit is a simple and efficient system for purification of total RNA from mammalian cultured cells, tissue, human blood cells, bacteria, and yeast.
291.00€ - 1226.00€
Specifications
| Isolation Technology | Silica Spin Column |
|---|---|
| Purification Time | 15 min. (After sample homogenization) |
| Elution Volume | 50–100 μL |
| Sample Type | Bacteria, Blood, Cells, Tissue, Yeast, Insects, Enzymatic Reactions |
| Final Product Type | Total RNA |
| Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
|---|---|---|---|---|---|---|---|---|---|
| Product Code | Brand | Quantity | Price | Quantity & Availability | |||||
|
10171080
|
Thermo Scientific™
K0732 |
250 Preps |
1226.00€
Each |
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|
10558360
|
Thermo Scientific™
K0731 |
50 Preps |
291.00€
Each |
Please sign in to purchase this item. Need a web account? Register with us today! | |||||
Description
Thermo Scientific GeneJET RNA Purification Kit is a simple and efficient system for purification of total RNA from mammalian cultured cells, tissue, human blood cells, bacteria, and yeast.
The kit utilizes a silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious cesium chloride gradients, alcohol precipitation, or toxic phenol-chloroform extractions.
RNA molecules longer than 200 nucleotides can be isolated with the GeneJET RNA Purification Kit in 15 minutes after the lysis step. The high-quality purified RNA can be used in a wide range of downstream applications, including RT-PCR, RT-qPCR, Northern blotting, and other RNA-based analyses.
Highlights
• Universal—can be used for both cell and tissue samples from a wide range of sources: mammalian blood, mammalian cell cultures, mammalian tissues, insect, yeast, and bacteria
• Efficient—high yields of total RNA.
• Fast—15 minutes after lysis step
• Pure—an A260/280 ratio > 1.9
• Convenient—spin columns are capped and assembled with collection tubes
Applications
Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including:
• RT-PCR
• RT-qPCR
• Northern blotting
• Nuclease protection assay
Includes
Proteinase K Lysis Buffer, Wash Buffer 1 (concentrated), Wash Buffer 2 (concentrated), Nuclease-free Water, GeneJET RNA Purification Columns (pre-assembled with Collection Tubes), Collection Tubes (2 mL), Collection Tubes (1.5 mL), Detailed Protocol
Specifications
| Silica Spin Column | |
| 50–100 μL | |
| Total RNA | |
| RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling | |
| Room Temperature | |
| Up to 160 μg |
| 15 min. (After sample homogenization) | |
| Bacteria, Blood, Cells, Tissue, Yeast, Insects, Enzymatic Reactions | |
| Not High-throughput Compatible (Manual) | |
| Mini | |
| Bacteria: ≤109 cells Blood: 0.5 mL Cells: ≤107 Tissue and Insects: 30 mg Yeast: ≤4 x 108 cells Enzymatic reactions: ≤100 μL |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- GeneJET RNA Purification Kit
Signal Word
- Danger
Hazard Category
- Acute toxicity Category 4
- LONG-TERM AQUATIC HAZARD Chronic 3
- Serious eye damage/eye irritation Category 2
- Respiratory sensitiser Category 1
- Skin corrosion/irritation Category 1 C
- Skin corrosion/irritation Category 2
Hazard Statement
- H302-Harmful if swallowed.
- H314-Causes severe skin burns and eye damage.
- H315-Causes skin irritation.
- H317-May cause an allergic skin reaction.
- H319-Causes serious eye irritation.
- H412-Harmful to aquatic life with long lasting effects.
Precautionary Statement
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P264-Wash hands thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P273-Avoid release to the environment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P284-¢In case of inadequate ventilation! wear respiratory protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P330-Rinse mouth.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P337+P313-If eye irritation persists: Get medical advice/attention.
- P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
- P362+P364-Take off contaminated clothing and wash it before reuse.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : Thiocyanic acid, Guanidine hydrochloride, Proteinase
For Research Use Only. Not for use in diagnostic procedures.