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  5. Thermo Scientific™ GeneJET RNA Purification Kit
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Thermo Scientific™ GeneJET RNA Purification Kit

Thermo Scientific GeneJET RNA Purification Kit is a simple and efficient system for purification of total RNA from mammalian cultured cells, tissue, human blood cells, bacteria, and yeast.

Brand:  Thermo Scientific™ K0732

796.90 EUR valid until 2024-11-30
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Product Code. 10171080

  • 1226.00€ / Each

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Description

Description

Thermo Scientific GeneJET RNA Purification Kit is a simple and efficient system for purification of total RNA from mammalian cultured cells, tissue, human blood cells, bacteria, and yeast.

The kit utilizes a silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious cesium chloride gradients, alcohol precipitation, or toxic phenol-chloroform extractions.

RNA molecules longer than 200 nucleotides can be isolated with the GeneJET RNA Purification Kit in 15 minutes after the lysis step. The high-quality purified RNA can be used in a wide range of downstream applications, including RT-PCR, RT-qPCR, Northern blotting, and other RNA-based analyses.

Highlights
• Universal—can be used for both cell and tissue samples from a wide range of sources: mammalian blood, mammalian cell cultures, mammalian tissues, insect, yeast, and bacteria
• Efficient—high yields of total RNA.
• Fast—15 minutes after lysis step
• Pure—an A260/280 ratio > 1.9
• Convenient—spin columns are capped and assembled with collection tubes

Applications
Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including:
• RT-PCR
• RT-qPCR
• Northern blotting
• Nuclease protection assay

Includes
Proteinase K Lysis Buffer, Wash Buffer 1 (concentrated), Wash Buffer 2 (concentrated), Nuclease-free Water, GeneJET RNA Purification Columns (pre-assembled with Collection Tubes), Collection Tubes (2 mL), Collection Tubes (1.5 mL), Detailed Protocol

TRUSTED_SUSTAINABILITY
Specifications

Specifications

• 5 x 600 μL Proteinase K; store at room temperature until opened, -20°C after opening
• 200 mL Lysis Buffer; room temperature
• 200 mL Wash Buffer 1 (concentrated); room temperature
• 100 mL Wash Buffer 2 (concentrated); room temperature
• 125 mL Nuclease-free Water; room temperature
• 250 GeneJET RNA Spin Columns with Collection Tubes; room temperature
• 250 Collection Tubes (2 mL); room temperature
• 250 Collection Tubes (1.5 mL); room temperature
15 min. (After sample homogenization)
Bacteria, Blood, Cells, Tissue, Yeast, Insects, Enzymatic Reactions
Not High-throughput Compatible (Manual)
250 Preps
Room Temperature
Up to 160 μg
Silica Spin Column
50–100 μL
Total RNA
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Mini
Bacteria: ≤109 cells
Blood: 0.5 mL
Cells: ≤107
Tissue and Insects: 30 mg
Yeast: ≤4 x 108 cells
Enzymatic reactions: ≤100 μL
Product Suggestions

Product Suggestions

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Safety and Handling

Safety and Handling

Product Identifier
  • GeneJET RNA Purification Kit

Signal Word
  • Danger

Hazard Category
  • Acute toxicity Category 4
  • LONG-TERM AQUATIC HAZARD Chronic 3
  • Serious eye damage/eye irritation Category 2
  • Respiratory sensitiser Category 1
  • Skin corrosion/irritation Category 1 C
  • Skin corrosion/irritation Category 2

Hazard Statement
  • H302-Harmful if swallowed.
  • H314-Causes severe skin burns and eye damage.
  • H315-Causes skin irritation.
  • H317-May cause an allergic skin reaction.
  • H319-Causes serious eye irritation.
  • H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P264-Wash hands thoroughly after handling.
  • P270-Do not eat, drink or smoke when using this product.
  • P273-Avoid release to the environment.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P284-¢In case of inadequate ventilation! wear respiratory protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P330-Rinse mouth.
  • P332+P313-If skin irritation occurs: Get medical advice/attention.
  • P337+P313-If eye irritation persists: Get medical advice/attention.
  • P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
  • P362+P364-Take off contaminated clothing and wash it before reuse.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information
  • MIXTURE LIST-Contains : Thiocyanic acid, Guanidine hydrochloride, Proteinase

SDS
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For Research Use Only. Not for use in diagnostic procedures.