Invitrogen™ CellBlox™ Blocking Buffer

Description

• Formulated to block nonspecific binding of Invitrogen™ NovaFluor™ labels with cells that can result in higher background labeling
• CellBlox™ Blocking Buffer is a non-antibody, non-protein–based blocking solution, and should be used every time a NovaFluor dye is used for labeling any cell type to minimize background labeling
• Recommended for use with cyanine-based dyes or cyanine-based tandem dyes to block non-specific interactions with monocytes and macrophages to minimize background labeling
• Use of CellBlox Blocking Buffer requires minimal change to most flow cytometry staining protocols
• Add 5 μL of buffer directly to a cell suspension containing 10³ to 10⁸ cells prior to the addition of an antibody, with 100 μL as a final staining volume
• Buffer may be added to an antibody cocktail mixture prior to labeling cells, by adding 5 μL of buffer for every stained sample to be labeled with the antibody cocktail mixture, with 100 μL as a final staining volume
• Always use CellBlox Blocking Buffer with NovaFluor dyes when labeling cells for best background reduction
• Compatible with all fluorophores and with Invitrogen™ LIVE/DEAD™ Fixable Dead Cell Stains
• Can be used with any fluorophore-antibody conjugate as a high-performance monocyte and macrophage blocking solution
• CellBlox Blocking Buffer is compatible with other blocking reagents such as Fc Block, blocking proteins, Brilliant Stain Buffer, and Super Bright Complete Staining Buffer
• Buffer is not required when labeling antibody-capture beads