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IBA Lifesciences CD8 Fab-TACS™ Agarose Column Starter Kit
Label-free isolation of CD8+ T cells
Brand: IBA Lifesciences 6-3305-002
This item is not returnable.
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Description
- Fab-TACS™ - Traceless Affinity Cell Selection - is a positive cell isolation approach based on our Strep-tag™ technology.
- Strep-Tactin™ TACS Agarose columns are filled with Strep-Tactin™ coated agarose macrobeads developed for cell isolation. Fab fragments fused to a Twin-Strep-tag™ (Fab-Streps) bind to the matrix.
- Target cells adhere to the matrix based on the exclusive binding of the Fab-Strep to a surface marker. Non-target cells are efficiently washed away.
- In a final step, the addition of biotin causes the elution of the target cells and the Fab-Streps.
- After elution, the Fab-Streps self-dissociate from the cell surfaces due to their reduced affinity.
- The label-free authentic target cells are now ready for further downstream applications
Specifications
| Strep-Tactin™ TACS Agarose columns, 0.3 mL (2 x), CD8 Fab-Strep (mouse), 10 x Buffer CI, Biotin stock solution, TACS Column Adapter | |
| Murine splenocytes | |
| Mouse | |
| 10 x Buffer Cl |
For Research Use Only.