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Invitrogen™ BLC/CXCL13 Mouse ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ EMCXCL13

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Product Code. 15455253

  • 628.00€ / 1 set
Estimated Shipment: 06-12-2024
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Description

Description

The Mouse BLC/CXCL13 ELISA quantitates Ms BLC in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms BLC. Principle of the method The Mouse BLC solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

B lymphocyte chemoattractant, independently cloned and named Angie, is a CXC chemokine strongly expressed in the follicles of the spleen, lymph nodes, and Peyer's patches. It preferentially promotes the migration of B lymphocytes (compared to T cells and macrophages), apparently by stimulating calcium influx into, and chemotaxis of, cells expressing Burkitt's lymphoma receptor 1 (BLR-1). It may therefore function in the homing of B lymphocytes to follicles.
TRUSTED_SUSTAINABILITY
Specifications

Specifications

O55038
2 pg/mL
BLC (CXCL13) ELISA Kit, 96 Assays, 1 Kit, Mouse Species, HRP Conjugate, Colorimetric Detection Method, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of BLC (CXCL13) in Samples Using a Microplate Reader
Solid-phase sandwich ELISA
Colorimetric
ELISA Kit
Plasma, Serum, Supernatant
96 Assays
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
55985
Mouse
<10%
HRP
RUO
Plasma, 5 μL; Serum, 5 μL; Supernatant, 100 μL
4631412M08Rik, ANGIE2, Angie, BCA-1, BLC, BLR1L, Scyb13
1 hr. 20 min.
1.37-1000 pg/mL
2pg/mL to 2000pg/mL
BLC (CXCL13), mouse BLC
Biotin
Sandwich ELISA Kit
Chemokine & Receptors
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL- 4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Mouse
Colorimetric Microplate Reader
Cxcl13
<12%
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Anti-mouse BLC precoated 96-well strip plate, lyophilized recombinant mouse BLC standard, 20X wash buffer, biotinylated antibody reagent, streptavidin-HRP reagent, TMB substrate, stop solution, contains 0.2M sulfuric acid, assay diluent (5X concentrated b
96 Tests
Inflammation
2°C to 8°C
Mouse
4 hr. 45 min.
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