missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Thermo Scientific™ 1-Step Human High-Yield IVT Kit
Express functional proteins with 10 to 100 times greater yield per mL than other mammalian IVT systems with Thermo Scientific™ 1-Step Human High-Yield In Vitro Translation (IVT) Kits.
Brand: Thermo Scientific™ 88892
This item is not returnable.
View return policy
Description
The Thermo Scientific™ 1-Step Human High-Yield In Vitro Translation (IVT) Kits with continuous-feed device enables the expression of functional proteins with 10 to 100 times greater yield per mL than other eukaryotic IVT translation systems.
The 1-Step High-Yield IVT System uses modified HeLa cell extracts to take advantage of the robust human transcription and translation machinery and generate functional full-length proteins. In this system, protein expression is performed in a proprietary mini dialysis device that allows a continuous supply of nucleotides, amino acids and energy generating substrates into the reaction while removing inhibitors of proteins synthesis. This continuous-exchange cell-free (CECF) system enables protein expression for up to 24 hours and produces protein yields ranging from 250 to 750μg/mL. The complete kits come with all components required for proper transcription and translation of recombinant protein, including an optimized expression vector.Highlights:
High expression – up to 750μg/mL of expressed protein
Reproducibility – low variability between experiments
Fast – express high levels of protein with an overnight incubation
Functional – obtain more active protein than with bacterial protein expression systems
1-Step Protein Expression:
Streamlined reaction – requires only five components
Easy-to-use – transcription and translation is performed in one reaction step
Adapt for labeling – amenable to incorporation of heavy or unnatural amino acids
Includes:
Complete kits contain cell lysate, reaction mix, accessory proteins, 5X dialysis buffer, control DNA, cloning vector, mini dialysis devices and centrifuge tubes
Requires:
30°C incubator or water bath
Recommended for:
Production of recombinant proteins for structural analysis; Generate active proteins for protein interaction studies; Perform rapid expression and characterization of mutant proteins; Small-scale production of virual particles; Incorporation of unnatural amino acids or toxic protein production
Specifications
| 1-step Human High-yield Maxi IVT Kit | |
| 2 x 2 mL | |
| Cell-Free |
| 2 Reactions of 2 μL Each | |
| Sufficient For: 2 reactions of 2 mL each • HeLa Lysate, 4 x 500 μL • Accessory Proteins, 4 x 100 μL • Reaction Mix, 4 x 200 μL • 5X Dialysis Buffer, 23 mL • Positive Control DNA (pCFE-GFP), 10 μg • pT7CFE1-NHis-GST-CHA, 10 μg • Maxi dialysis Device, 2 each • Nuclease-free Water, 3 x 50 mL Store micro-dialysis devices and nuclease-free water at room temperature. Store all other components at -80°C. |
|
| Insoluble Protein |
Product Content Correction
Your input is important to us. Please complete this form to provide feedback related to the content on this product.
Product Title
For Research Use Only. Not for use in diagnostic procedures.
Spot an opportunity for improvement?Share a Content Correction