Protein Analysis Reagents
Protein Analysis Reagents
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Thermo Scientific™ Zeba™ Spin Desalting Columns, Plates, and Cartridges, 7K MWCO, 0.5–100 mL
Perform high-throughput protein recovery (7K MWCO) and sample desalting for mass spectrometry, HPLC, capillary electrophoresis, metabolite screening, and assay development with ready-to-use Thermo Scientific Pierce Zeba desalting chromatography columns, plates, and cartridges.
MWCO | 7.0 kDa |
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Column Type | Size-exclusion, Proprietary Resin |
Purification Target | Buffer Exchange, Protein |
Product Line | Zeba™ |
Thermo Scientific™ NP-40 Surfact-Amps™ Detergent Solution
Thermo Scientific™ Surfact-Amps NP-40 Detergent Solution is highly-purified NP-40 detergent stabilized and supplied as a 10% solution.
Form | Liquid |
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Reagent Type | Detergent Solution |
Product Line | Surfact-Amps™ |
Cytiva Disposable PD-10 Desalting Columns
Use for desalting and buffer exchange, as well as removal of low-molecular weight compounds. Cytiva Life Sciences™ Disposable PD-10 Desalting Columns provide fast, high-recovery.
Protein Family | Avidin |
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Content And Storage | Upon receipt store product 4°C. Product is shipped at ambient temperature. |
Target Molecule | Biotin |
Form | Solid |
Label or Dye | Unlabeled |
Thermo Scientific™ SnakeSkin™ Dialysis Tubing
Perform desalting and buffer exchange for 10 to 100 mL samples with this 3.5K, 7K, and 10K molecular weight cut-off (MWCO), ready-to-use form of traditional dialysis membrane tubing. Securely close dialysis tubing up to 50 mm flat diameter with the sturdy, non-floating tube SnakeSkin Dialysis Clips.
Product Line | SnakeSkin™ |
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Invitrogen™ Dynabeads™ Protein A for Immunoprecipitation
Dynabeads Protein A beads are uniform, 2.8-μm superparamagnetic beads with recombinant Protein A (∼45 kDa) covalently coupled to the surface.
Shipping Condition | Ambient temperature |
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Content And Storage | Dynabeads Protein A are supplied in PBS, pH 7.4 w/0.01% Tween-20, 0.09% NaN3 Store at 2–8°C. |
Purity or Quality Grade | For Research Use Only |
Description | Recombinant Protein A covalently bound to Dynabeads |
Surface Functionality | Protein A |
Material | Polystyrene |
Concentration | 30 mg/mL |
For Use With (Application) | Immunoprecipitation |
High-throughput Compatibility | High-throughput Compatible |
Ligand Type | Protein A |
Type | Superparamagnetic Beads |
Target | Antibodies |
Format | Beads in suspension |
For Use With (Equipment) | KingFisher™ Sample Purification System, DynaMag™ magnets |
Diameter (Metric) | 2.8 μm |
Certifications/Compliance | ISO9001 and ISO13485 |
Uniformity | Monosized 2.8 mm (CV <5%) |
Product Line | Dynabeads™ |
Thermo Scientific™ Slide-A-Lyzer™ G3 Dialysis Cassettes, 3.5K MWCO
Thermo Scientific Slide-A-Lyzer™ G3 Dialysis Cassettes facilitate the rapid and trouble-free dialysis of sample volumes from 0.5 to 125 mL. Unlike standard flat tubing, these innovative devices do not require knots or clips that can lead to leaking and sample loss.
MWCO | 3.5 kDa |
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Format | Cassette |
Membrane | Cellulose Membrane, Dialysis |
Green Features | Less waste |
Purification Target | Buffer Exchange, Protein |
Product Line | Slide-A-Lyzer™ |
Thermo Scientific™ Pierce™ Streptavidin
Purified recombinant streptavidin enables detection of biotinylated antibodies and other probes in a variety of standard assay methods including western blotting, ELISA, immunohistochemistry, and fluorescence imaging.
Content And Storage | Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 2 to 8°C. |
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Conjugate | Unconjugated |
Target Molecule | Biotin |
Product Line | Pierce™ |
Thermo Scientific™ Pierce™ BCA Protein Assay Kits
The Pierce BCA Protein Assay Kit is a high-precision, detergent-compatible protein assay for determination of protein concentration. Pierce BCA reagents provide accurate determination of protein concentration with most sample types encountered in protein research.
Assay | BCA Assay |
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For Use With (Equipment) | Spectrophotometer, Microplate Reader |
Product Type | Protein Quantitation Assay |
For Use With (Application) | Solution-based Detection, Absorbance |
Product Line | Pierce™ |
Detection Method | Colorimetric |
Cytiva HisTrap™ HP Prepacked Columns
Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
Flow Rate | 4 mL/min. (1 mL), 5 mL/min. (5 mL) |
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Type | Pre-packed Column |
Content And Storage | 4°C to 30°C |
Description | HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery. |
Max. Operating Pressure | 5 bar (0.5 MPa, 70 psi) |
pH Range | 3 to 12 |
Material | Polypropylene |
Support Type | 6% Highly Cross-linked Agarose |
Thermo Scientific™ Pierce™ Horseradish Peroxidase
Purified horseradish peroxidase enzyme for use in activity assays and conjugation to antibodies for ELISA, Western blot and immunohistochemistry applications.
Content And Storage | Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, but do not store above -20°C. |
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Form | Powder |
Label or Dye | HRP (Horseradish Peroxidase) |
For Use With (Application) | Western Blotting, ELISA, Immunohistochemistry |
Product Line | Pierce™ |
Thermo Scientific™ NHS-Fluorescein (5/6-carboxyfluorescein succinimidyl ester), mixed isomer
Activated derivatives of green-fluorescent fluorescein dye to label antibodies, proteins and other molecules for fluorescence imaging (ex 494; em 518).
Content And Storage | Store desiccated at -20°C, protect from moisture |
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Product Type | NHS-Fluorescein |
Label or Dye | FITC (Fluorescein) |
Reactive Moiety | Active Ester, Succinimidyl Ester, NHS Ester |
Solubility | DMF (Dimethylformamide) |
Emission | 518 |
Excitation | 494 |
Label Type | Classic Dyes |
Chemical Reactivity | Amine |
Product Line | Pierce™ |
Invitrogen™ Dynabeads™ M-270 Epoxy
Dynabeads™ M-270 Epoxy beads are 2.8 μm superparamagnetic beads containing surface epoxy groups.
Shipping Condition | Room Temperature |
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Type | Epoxy Bead |
Purity or Quality Grade | Research Grade |
Format | Freeze Dried |
Surface Functionality | Epoxy |
Sample Type | Any Sample Type |
Diameter (Metric) | 2.8 μm |
Shelf Life | 24 months from date of manufacture |
Elution Volume Min | 5 μL |
Product Line | DYNAL™, Dynabeads™ |
Thermo Scientific™ TMTpro™ 16plex Label Reagent Set
The Thermo Scientific TMTpro 16plex Label Reagent Set enables multiplexing of up to 16 samples for multiplexed protein identification and quantitation using tandem mass spectrometry (MS/MS).
Workflow Step | Protein Labeling |
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Final Product Type | Peptide |
Product Type | Isobaric Label Reagent Set |
For Use With (Equipment) | Mass Spectrometer |
Label or Dye | Tandem Mass Tag™ (TMT) Labels |
Detection Method | Mass Spectrometry |
Product Line | TMT™ |
Thermo Scientific™ NHS-Rhodamine (5/6-carboxy-tetramethyl-rhodamine succinimidyl ester), mixed isomer
Label antibodies, proteins and other molecules for fluorescence imaging (ex 546; em 572-615) using these activated derivatives of red-fluorescent rhodamine dye.