Search results for "pcr reagents"

Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents
AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.

Shipping Condition | Dry Ice |
---|---|
GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 25 X |
Description | Works on all AB platforms, but is optimized for the 7500. |
Reverse Transcriptase | ArrayScript™ |
Concentration | 25X |
PCR Method | 1-step RT-qPCR |
Detection Method | Primer-probe |
Polymerase | AmpliTaq Gold DNA Polymerase |
Format | Tube |
Product Type | One-Step RT-PCR Reagent |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System |
Sample Type | RNA |
Reaction Speed | Standard |
Product Line | AgPath-ID™, Ambion™ |
Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents with manual
AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.
GC-Rich PCR Performance | High |
---|---|
Fidelity (vs. Taq) | 25 X |
Description | Works on all AB platforms, but is optimized for the 7500. |
Reverse Transcriptase | ArrayScript™ |
Concentration | 25X |
PCR Method | 1-step RT-qPCR |
Detection Method | Primer-probe |
Polymerase | AmpliTaq Gold DNA Polymerase |
Format | Tube |
Product Type | One-Step RT-PCR Reagent |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7500 System |
Reaction Speed | Standard |
Product Line | AgPath-ID™, Ambion™ |
Thermo Scientific™ PCR Master Mix (2X)
Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers.

Polymerase | Taq DNA Polymerase |
---|---|
GC-Rich PCR Performance | Low |
Fidelity (vs. Taq) | 1X |
Reaction Format | SuperMix or Master Mix |
Product Type | PCR Master Mix |
Reaction Speed | Standard |
Hot Start | No |
Overhang | 3'-A |
Concentration | 2X |
Starting Material | DNA |
For Use With (Application) | Standard PCR |
Invitrogen™ Platinum™ Direct PCR Universal Master Mix
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This product offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
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Invitrogen Platinum Direct PCR Universal Master Mix is designed to amplify DNA directly from various samples without the need to purify DNA.

Shipping Condition | Dry Ice |
---|---|
Polymerase | Platinum II Taq Hot-Start DNA Polymerase |
GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 1X |
Size (Final Product) | 8 kb |
Reaction Format | SuperMix or Master Mix |
Product Type | Direct PCR Universal Master Mix |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | 3'-A |
Green Features | Less hazardous, less waste |
Concentration | 2X |
For Use With (Application) | Hot-start PCR,Real Time PCR (qPCR) |
Thermo Scientific™ Phusion High-Fidelity PCR Kit
Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.
Shipping Condition | Dry Ice |
---|---|
Polymerase | Phusion High-Fidelity DNA Polymerase |
GC-Rich PCR Performance | High |
Content And Storage | • Phusion DNA Polymerase (2 U/μL) • 5X Phusion GC & HF Buffer • dNTP mix (10 mM each) • Control template (λ DNA) and DNA size standard • 1.3 kb & 10 kb primers (4 μM each) • 50 mM MgCl2 solution and DMSO Store at –20°C. |
Fidelity (vs. Taq) | 52X |
Size (Final Product) | 20 kb or less |
Reaction Format | Separate Components |
Product Type | High-Fidelity PCR Kit |
Reaction Speed | Fast |
Hot Start | No |
Overhang | Blunt |
For Use With (Application) | Standard PCR,High-fidelity PCR |
Invitrogen™ SuperScript™ IV One-Step RT-PCR System
The Invitrogen SuperScript One-Step RT-PCR System, available with or without ezDNase, combines the high processivity of SuperScript IV Reverse Transcriptase (RT) with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched one-step RT-PCR performance.

Shipping Condition | Dry Ice |
---|---|
GC-Rich PCR Performance | High |
Reverse Transcriptase | SuperScript IV |
Final Product Type | PCR Amplified cDNA |
Quantity | Each |
PCR Method | 1-step RT-PCR |
Detection Method | Gel Electrophoresis |
Polymerase | Platinum SuperFi |
Format | Kit |
Size (Final Product) | Up to 14 kb |
Reaction Format | Master Mix |
Reaction Speed | 10 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Hot Start | Built-In Hot Start |
Optimal Reaction Temperature | 45°C to 60°C |
Technique | 1-step RT-PCR |
Starting Material | RNA |
Thermo Scientific™ Phusion Blood Direct PCR Kit
Thermo Scientific Phusion Blood Direct PCR Kit is developed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR.
Shipping Condition | Wet or Dry Ice |
---|---|
Polymerase | Phusion Blood II DNA Polymerase |
GC-Rich PCR Performance | High |
Reaction Format | Separate Components |
Product Type | Direct PCR Kit |
Sample Type | Blood |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Concentration | 2 U/μL |
Starting Material | DNA |
PCR Method | Direct PCR |
Applied Biosystems™ TaqMan™ Reverse Transcription Reagents
TaqMan Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)16, and reverse primers are included for cDNA synthesis flexibility.
Shipping Condition | Dry Ice |
---|---|
Format | Kit |
Reverse Transcriptase | MultiScribe |
Size (Final Product) | Up to 5 kb |
Reaction Format | Separate components |
Final Product Type | First-Strand cDNA |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Yes |
Concentration | 50 U/μL |
Optimal Reaction Temperature | 42°C |
Technique | Reverse Transcription |
Starting Material | RNA |
For Use With (Application) | Real Time PCR (qPCR) |
Applied Biosystems™ True Allele™ PCR Premix
Applied Biosystems True Allele PCR Premix is a PCR master mix that contains AmpliTaq Gold DNA Polymerase, dNTPs, MgCl2, and buffer.
Thermo Scientific SureTect™ Listeria species PCR Assay
Rapidly detect Listeria species in food and environmental samples with the Thermo Scientific™ SureTect™ Listeria Species PCR Assay.
Applied Biosystems™ TaqMan™ Sample-to-SNP™ Kit (20mL Sample prep, 50mL PCR)
Go from Biological Sample to Genotyping Results in less than one hour.
Thermo Scientific™ KingFisher™ Flex Purification System, KingFisher with 96 PCR head
Prepare samples efficiently from a variety of materials with Thermo Scientific™ KingFisher™ Flex Magnetic Particle Processors.
Invitrogen™ Lyo-ready Enzymes and Reagents for Recombinase Polymerase Amplification (RPA)
Invitrogen Lyo-ready RPA reagents are designed for fast, sensitive, and specific DNA amplification through isothermal recombinase polymerase amplification (RPA) reactions. Lyo-ready RPA reagents are available in stand-alone and kit formats.
Shipping Condition | Dry Ice |
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Form | Liquid |
Research Category | Nucleic Acid Amplification |
Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase
The SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the sensitive, reproducible, end-point detection and analysis of RNA molecules by RT-PCR.
Shipping Condition | Dry Ice |
---|---|
GC-Rich PCR Performance | High |
Reverse Transcriptase | SuperScript III |
Final Product Type | PCR Amplified cDNA |
PCR Method | 1-step RT-PCR |
Detection Method | Gel Electrophoresis |
Polymerase | Platinum Taq DNA Polymerase |
Format | One-Step RT-PCR System |
Size (Final Product) | Up to 4.5 kb |
Reaction Format | Premixed Components |
Reaction Speed | 30 min. |
Reagent Type | Reverse Transcription |
Ribonuclease H Activity | Reduced |
Hot Start | Built-In Hot Start |
Optimal Reaction Temperature | 50°C |
Technique | 1-Step RT-PCR |
Starting Material | RNA |
Invitrogen™ SuperScript IV UniPrime One-Step RT-PCR System
Invitrogen SuperScript IV UniPrime One-Step RT-PCR System is a convenient one-step formulation for performing fast cDNA synthesis and PCR amplification in a single tube.
GC-Rich PCR Performance | High |
---|---|
Format | 1-step RT-PCR Kit |
Reverse Transcriptase | SuperScript IV |
Size (Final Product) | Up to 13 kb |
Reaction Format | Master Mix |
Reagent Type | Reverse Transcription |
Hot Start | Built-in Hot Start |
Optimal Reaction Temperature | 50°C to 65°C |
Technique | 1-step RT-PCR |
Starting Material | RNA |
PCR Method | 1-step RT-PCR |
Multiplex Capability | Yes |
Detection Method | Gel Electrophoresis |