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Thermo Scientific™ PCR Master Mix (2X)
Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers.
Specifications
| GC-Rich PCR Performance | Low |
|---|---|
| Polymerase | Taq DNA Polymerase |
| Reaction Speed | Standard |
| Product Type | PCR Master Mix |
| For Use With (Application) | Standard PCR |
| Product Code | Brand | No. of Reactions | Price | Quantity & Availability | |||||
|---|---|---|---|---|---|---|---|---|---|
| Product Code | Brand | No. of Reactions | Price | Quantity & Availability | |||||
|
11853913
|
Thermo Scientific™
K0171 |
200 Reactions |
N/A
|
N/A | |||||
|
11863913
|
Thermo Scientific™
K0172 |
1000 Reactions |
N/A
|
N/A
|
|||||
Description
Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimized for efficient and reproducible PCR.
Highlights
- Convenient, ready-to-use mix
- Thermostable—half life is more than 40 min. at 95°C
- Generates PCR products with 3'-dA overhangs
- Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)
Applications
- Routine PCR amplification of DNA fragments up to 5 kb
- High throughput PCR
- DNA labeling
Note
The error rate of Taq DNA Polymerase in PCR is 2.2 x 10-5 errors per nt per cycle, as determined by a modified method described in. Accordingly, the accuracy of PCR is 4.5 x 104. Accuracy is an inverse of the error rate and shows an average number of correct nucleotides incorporated before an error occurs.
Specifications
| Low | |
| Standard | |
| Standard PCR | |
| 1X | |
| 3'-A | |
| DNA |
| Taq DNA Polymerase | |
| PCR Master Mix | |
| 2X | |
| No | |
| SuperMix or Master Mix |
For Research Use Only. Not for use in diagnostic procedures.