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Protein Purification

Protein Purification

Kits, columns, gels, reagents, enzymes, inhibitors, and other assorted products for use with protein purification procedures.
Protein Purification Reagents and Kits (1,551)
Magnetic Affinity Separation Particles (242)
Protease Inhibitors and Phosphatase Inhibitors (178)
Gel Filtration Media and Columns (113)
Spin Columns and Gravity-flow Columns (112)
Miscellaneous Immobilization Supports (110)
Cell Lysis Detergents (88)
In vitro Protein Expression Systems (43)
Affinity Gels (35)
Biotin-Binding Affinity Resins and Beads (33)
Miscellaneous Immobilized Affinity Ligands (26)
Cell Lysis Enzymes (19)

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1 – 15 of 1,624 results
1

Cytiva Gel Filtration Calibration Kits

Calibrate gel filtration columns simply, reliably. Cytiva Life Sciences™ Gel Filtration Calibration Kits contain a well-defined series of protein standards.

Promotions are available
EDGE
Content And Storage 2° to 8°C
Product Type Calibration Kit
For Use With (Equipment) High-resolution media and prepacked column
Quantity Each
For Use With (Application) Calibration of high-resolution gel filtration media and column such as Superdex™, Superose and Sephacryl
2

Cytiva Superose™ 6 Increase Columns

High-resolution size-exclusion chromatography for studying large proteins and complexes. Cytiva Lifescience™ Superose™ 6 Increase Columns delivers high quality in size exclusion chromatography (SEC).

Promotions are available
EDGE
Particle Size 8.6μm
Ligand Type Cross-linked agarose
Content And Storage 4° to 30° in 20% ethanol
pH Range 3 to 12
Column Type Gel Filtration Column
Material Borosilicate glass
Max. Pressure 435 psi
3

Cytiva Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns

For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. Cytiva Lifescience™ Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification.

Promotions are available
4

Cytiva His GraviTrap™ Columns

Use for purification of histidine-tagged proteins using gravity flow. His GraviTrap™ Columns enable fast and simple purification.

EDGE
5

Cytiva HiTrap Capto S ImpAct Prepacked Columns

HiTrap Capto S ImpAct columns are prepacked with Capto S ImpAct cation exchange chromatography medium for screening and small-scale protein purifications using ion exchange chromatography (IEX).

Promotions are available
Particle Size 50μm
pH Range 4 to 12
Product Type HiTrap Capto S ImpAct Prepacked Column
Column Type Ion Exchange Column
Material High-flow agarose
6

Thermo Scientific™ NP-40 Surfact-Amps™ Detergent Solution

Thermo Scientific™ Surfact-Amps NP-40 Detergent Solution is highly-purified NP-40 detergent stabilized and supplied as a 10% solution.

EDGE
Form Liquid
Reagent Type Detergent Solution
Product Line Surfact-Amps™
7

Invitrogen™ Dynabeads™ Protein A for Immunoprecipitation

Dynabeads Protein A beads are uniform, 2.8-μm superparamagnetic beads with recombinant Protein A (∼45 kDa) covalently coupled to the surface.

EDGE
Shipping Condition Ambient temperature
Content And Storage Dynabeads Protein A are supplied in PBS, pH 7.4 w/0.01% Tween-20, 0.09% NaN3 Store at 2–8°C.
Purity or Quality Grade For Research Use Only
Description Recombinant Protein A covalently bound to Dynabeads
Surface Functionality Protein A
Material Polystyrene
Concentration 30 mg/mL
For Use With (Application) Immunoprecipitation
High-throughput Compatibility High-throughput Compatible
Ligand Type Protein A
Type Superparamagnetic Beads
Target Antibodies
Format Beads in suspension
For Use With (Equipment) KingFisher™ Sample Purification System, DynaMag™ magnets
Diameter (Metric) 2.8 μm
Certifications/Compliance ISO9001 and ISO13485
Uniformity Monosized 2.8 mm (CV <5%)
Product Line Dynabeads™
8

Cytiva HisTrap™ HP Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

EDGE
Flow Rate 4 mL/min. (1 mL), 5 mL/min. (5 mL)
Type Pre-packed Column
Content And Storage 4°C to 30°C
Description HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery.
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Material Polypropylene
Support Type 6% Highly Cross-linked Agarose
9

Invitrogen™ Dynabeads™ M-270 Epoxy

Dynabeads™ M-270 Epoxy beads are 2.8 μm superparamagnetic beads containing surface epoxy groups.

Promotions are available
EDGE
Shipping Condition Room Temperature
Type Epoxy Bead
Purity or Quality Grade Research Grade
Format Freeze Dried
Surface Functionality Epoxy
Sample Type Any Sample Type
Diameter (Metric) 2.8 μm
Shelf Life 24 months from date of manufacture
Elution Volume Min 5 μL
Product Line DYNAL™, Dynabeads™
10

Cytiva PD MiniTrap™ G-25 Sample Preparation Columns

Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 0.5mL. Cytiva Life Sciences™ D MiniTrap™ G-25 Sample Preparation Columns are convenient and prepacked.

EDGE
11

Thermo Scientific™ Aprotinin Protease Inhibitor

Customize your protease inhibitor cocktail formulation with these small packages of individual protease inhibitor peptides and compounds.

EDGE
12

Invitrogen™ Dynabeads™ MyOne™ Carboxylic Acid

Surface carboxylic acid groups allow covalent amide bond formation to proteins/peptides via primary amino- or sulphydryl groups.

EDGE
Shipping Condition Room Temperature
Type Carboxylic Acid Magnetic Bead
Content And Storage Store in refrigerator (2–8°C).
Purity or Quality Grade Research Grade
Format Beads in solution
Surface Functionality Carboxylic Acid
Sample Type Any Sample Type
Diameter (Metric) 1 μm
For Use With (Application) Protein Purification and Isolation
High-throughput Compatibility High-throughput Compatible
Elution Volume Min 5 μL
Product Line Dynabeads™, MyOne™
13

Cytiva HisTrap™ FF Columns: 16 x 25mm

Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.

EDGE
Flow Rate <20 mL/min.
Type Pre-packed Column
Content And Storage 4°C to 30°C, 20% Ethanol
Diameter (English) Inner 0.6 in.
Description HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Diameter (Metric) Inner 16 mm
Material Polypropylene
Support Type 6% Cross-linked Agarose
14

Cytiva PreScission™ Protease

Use for site-specific separation of the GST tag from proteins expressed using pGEX-6P vectors. Cytiva Life Sciences™ PreScission™ Protease G allows simultaneous protease immobilization and cleavage of GST fusion proteins.

EDGE
15

Cytiva HiTrap™ Capto™ Adhere Columns

Designed to remove contaminants in post-protein A purification

EDGE
Particle Size 75μm
Buffer Aqueous Buffer
Ligand Type N-benzyl-n-methyl Ethanolamine
Height (Metric) 25mm
Content And Storage 4° to 30°C
pH Range 3 to 12
Product Type HiTrap Capto adhere column
Column Type Affinity Column
Material Polypropylene
For Use With (Application) Easy screening and convenient process development
Support Type Highly Cross-linked Agarose
Height (English) 0.98 in.