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Invitrogen™ TOP10 Electrocomp™ Kit
TOP10 Electrocomp E. coli cells are provided at a transformation efficiency of 1 x 1010 cfu/μg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation.
Brand: Invitrogen™ C66424
This item is not returnable.
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Description
TOP10 Electrocomp E. coli cells are provided at a transformation efficiency of 1 x 1010 cfu/μg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation. This strain is used in wide range of applications and allows stable replication of high-copy number plasmids and maintenance of large plasmids.
The TOP10 genotype is similar to the DH10B strain and features:
• hsdR for efficient transformation of unmethylated DNA from PCR amplifications
• mcrA for efficient transformation of methylated DNA from genomic preparations
• lacZΔM15 for blue/white color screening of recombinant clones
• endA1 for cleaner preparations of DNA and better results in downstream applications due to the elimination of non-specific digestion by Endonuclease I
• recA1 for reduced occurrence of non-specific recombination in cloned DNA
Notes
Please see the product manual for reaction scale recommendations. A high-voltage electroporation apparatus is required.
Genotype
F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL endA1 nupG
Find the strain and format that fits your needs
• TOP10 is available in chemically competent and electrocompetent cell formats.
• Choose MultiShot competent cells for high-throughput cloning applications.
Specifications
| High-efficiency cloning and plasmid propagation | |
| Contains enough electrocompetent cells for 75 x 40μL rxns or 150 x 20μL rxns | |
| 30 x 100 μL |
For Research Use Only. Not for use in diagnostic procedures.