missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Invitrogen™ TA Cloning™ Kit, Dual Promoter, with pCR™II Vector and One Shot™ INVαF' Chemically Competent E. coli
Provides quick, one-step cloning strategy for directly inserting a Taq-amplified PCR product into plasmid vector
Brand: Invitrogen™ K205001
316.96 EUR valid until 2024-11-30
Use promo code "21651" to get your promotional price.
This item is not returnable.
View return policy
Description
The T7 and Sp6 promoters of the pCRII vector allow in vitro transcription of the insert to produce sense or anti-sense products. The TA Cloning Kit Dual Promoter uses the pCRII cloning vector and ExpressLink T4 DNA Ligase to generate a ligation product in a fifteen-minute, room-temperature ligation step. Reactions typically yield >80% recombinants containing inserts.
- Fast and convenient: 15-minute, room-temperature ligation
- Efficient: blue/white screening and >80% clones with correct insert
- Flexible: choice of kanamycin or ampicillin resistance for flexible antibiotic selection
- Hassle-free: eliminates any enzymatic modifications of PCR product
- Streamlined: does not require use of PCR primers that contain restriction sites
- 3'-T overhangs for direct ligation of Taq-amplified PCR products
- T7 and Sp6 promoters for in vitro RNA transcription and sequencing
- Versatile polylinker with flanking EcoR I sites for easy excision of inserts
- M13 forward and reverse primer sites for sequencing
- How TA Cloning Works\
- Taq polymerase has non-template-dependent activity that adds single deoxyadenosine (A) to 3' ends of PCR products
- Linearized vector supplied in this kit has single 3' deoxythymidine (T) residues
- Allows PCR inserts to ligate efficiently with vector
- Kit Configurations
- TA Cloning Kit is offered in a variety of configurations: with One Shot INVF' Chemically Competent E. coli (K2050-01 and K2050-40), One Shot TOP10F' Chemically Competent E. coli (K2060-01 and K2060-40), and without competent cells (K2750-20 and K2750-40) in 20- and 40-reaction kit sizes
Cloning, PCR Cloning
Specifications
| INVαF ́ | |
| TA Cloning | |
| Kit | |
| Linearized pCRII vector, ExpressLink T4 DNA ligase, 5X ExpressLink T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, controls, One Shot INVαF' chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid. | |
| One Shot™ | |
| T7, SP6 |
| Chemically Competent E. coli | |
| This kit contains linearized pCR™II vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. Competent cell kits contain One Shot™ chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid. Store One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored. |
|
| PCR Cloning | |
| 20 Reactions | |
| Cloning Kit | |
| pCRII |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- TA Cloning Kit, Dual Promoter, with pCRII Vector and One Shot INV
Signal Word
- Warning
Hazard Category
- Reproductive toxicity Category 2
- Skin sensitiser Category 1
Hazard Statement
- H317-May cause an allergic skin reaction.
- H361-Suspected of damaging fertility or the unborn child.
Precautionary Statement
- P201-Obtain special instructions before use.
- P202-Do not handle until all safety precautions have been read and understood.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P272-Contaminated work clothing should not be allowed out of the workplace.
- P273-Avoid release to the environment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P308+P313-IF exposed or concerned: Get medical advice/attention.
- P333+P313-If skin irritation or rash occurs: Get medical advice/attention.
- P362+P364-Take off contaminated clothing and wash it before reuse.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : 2-Mercaptoethanol, Hydrogen chloride
For Research Use Only. Not for use in diagnostic procedures.