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Thermo Scientific™ T-REx™ Tango™ P2RY14-bla U2OS Cells

Brand: Thermo Scientific™ K1858

Product Code. 10185694

13050.00€ / Each

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Description

T-REx™ Tango™ P2RY14-bla U2OS cells contain the human purinergic receptor P2Y14 (P2RY14) under doxycycline inducible control linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase reporter gene under the control of a UAS response element. The T-REx™ Tango™ P2RY14-bla U2OS cells have been functionally validated for Z' factor and EC50 concentrations of UDP-glucose. In addition, T-REx™ Tango™ P2RY14-bla U2OS cells have been tested for assay performance under variable conditions.

Specifications

Accession Number	NM_014879.2
Cell State	Dividing Cells
Cytoplasmic Tail	Native Tail
For Use With (Application)	FRET
Population Status	Single Cell Clone Origin
Quantity	8 x 10⁶ cells/vial
Reporter Gene	BLA (Beta-Lactamase)
Type	U2OS Cell
System Type	Tango™

Assay Entry	Cell-based beta-arrestin recruitment
Content And Storage	Tango™ cell lines are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
Detection Method	Fluorescence
Human Protein Class	G-Protein Coupled Receptor (GPCR)
Product Line	Tango™
Readout	End Point
Target Entry	P2RY14
Cell Line	U2OS
Druggable Target	GPCR

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Thermo Scientific™ T-REx™ Tango™ P2RY14-bla U2OS Cells

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