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SuperSignal™ Western Blot Substrate Bundle, Femto + trial-size Pico PLUS
This substrate kit is comprised of SuperSignal West Femto Maximum Sensitivity Substrate plus a sample size of SuperSignal West Pico PLUS Chemiluminescent Substrate to provide chemiluminescent detection substrates for both high sensitivity and routine western blotting.
Brand: Thermo Scientific A43841
517.28 EUR valid until 2024-12-20
Use promo code "21651" to get your promotional price.
Description
SuperSignal West Femto Maximum Sensitivity Substrate
Thermo Scientific SuperSignal West Femto Maximum Sensitivity Substrate is an ultra-sensitive enhanced chemiluminescent (ECL) substrate for low femtogram protein level detection by western blot analysis with horseradish peroxidase (HRP) enzyme. It is optimized to detect low abundant and/or difficult to detect targets.
- Sensitivity: low-femtogram (mid-zeptomole)
- Stability: 8-hr working solution stability, 1-year kit stability at 4°C
- Compatibility: nitrocellulose and PVDF membranes
- Signal duration: up to 8 hours
- Recommended primary antibody concentration: 1:5,000–1:100,000 dilution (10–200 ng/mL)
- Recommended secondary antibody concentration: 1:100,000–1:500,000 dilution (2–10 ng/mL)
SuperSignal West Pico PLUS Chemiluminescent Substrate
Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate enables picogram to high femtogram-level protein detection by western blot analysis. It is best for routine, everyday western blotting applications, providing excellent signal intensity, sensitivity and duration across a wide range of targets.
- Sensitivity: pictogram to femtogram sensitivity
- Stability: 8-hr working solution stability; 1-year kit stability at room temperature
- Compatibility: nitrocellulose and PVDF membranes
- Signal duration: 6 to 24 hours
- Recommended primary antibody concentration: 1:1,000–1:5,000 dilution (0.2–1.0 μg/mL)
- Recommended secondary antibody concentration: 1:20,000–100,000 dilution (10–50 ng/mL)
Specifications
Substrate | |
Liquid | |
Nitrocellulose, PVDF | |
1 Bundle | |
Picogram to femtogram, low femtogram (mid-zeptomole) | |
• 200 mL SuperSgnal West Femto reagent, store at 2–8°C • 20 mL SuperSignal West Pico PLUS reagent, store at room temperature |
Chemiluminescence | |
Western Blot | |
SuperSignal™ | |
Horseradish Peroxidase | |
60 Mini-Gel Blots, 5000 sq. cm of membrane; 2 Mini-Gel Blots, 200 sq. cm of Membrane | |
Room Temperature |
For Research Use Only. Not for use in diagnostic procedures.