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SuperSignal™ Western Blot Substrate Bundle, Femto + trial-size Pico PLUS

This substrate kit is comprised of SuperSignal West Femto Maximum Sensitivity Substrate plus a sample size of SuperSignal West Pico PLUS Chemiluminescent Substrate to provide chemiluminescent detection substrates for both high sensitivity and routine western blotting.

Brand:  Thermo Scientific A43841

517.28 EUR valid until 2024-12-20
Use promo code "21651" to get your promotional price.


Product Code. 17153209

  • 848.00€ / 200mL
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Description

Description

SuperSignal West Femto Maximum Sensitivity Substrate

Thermo Scientific SuperSignal West Femto Maximum Sensitivity Substrate is an ultra-sensitive enhanced chemiluminescent (ECL) substrate for low femtogram protein level detection by western blot analysis with horseradish peroxidase (HRP) enzyme. It is optimized to detect low abundant and/or difficult to detect targets.

  • Sensitivity: low-femtogram (mid-zeptomole)
  • Stability: 8-hr working solution stability, 1-year kit stability at 4°C
  • Compatibility: nitrocellulose and PVDF membranes
  • Signal duration: up to 8 hours
  • Recommended primary antibody concentration: 1:5,000–1:100,000 dilution (10–200 ng/mL)
  • Recommended secondary antibody concentration: 1:100,000–1:500,000 dilution (2–10 ng/mL)

SuperSignal West Pico PLUS Chemiluminescent Substrate

Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate enables picogram to high femtogram-level protein detection by western blot analysis. It is best for routine, everyday western blotting applications, providing excellent signal intensity, sensitivity and duration across a wide range of targets.

  • Sensitivity: pictogram to femtogram sensitivity
  • Stability: 8-hr working solution stability; 1-year kit stability at room temperature
  • Compatibility: nitrocellulose and PVDF membranes
  • Signal duration: 6 to 24 hours
  • Recommended primary antibody concentration: 1:1,000–1:5,000 dilution (0.2–1.0 μg/mL)
  • Recommended secondary antibody concentration: 1:20,000–100,000 dilution (10–50 ng/mL)
TRUSTED_SUSTAINABILITY
Specifications

Specifications

Substrate
Liquid
Nitrocellulose, PVDF
1 Bundle
Picogram to femtogram, low femtogram (mid-zeptomole)
• 200 mL SuperSgnal West Femto reagent, store at 2–8°C
• 20 mL SuperSignal West Pico PLUS reagent, store at room temperature

Chemiluminescence
Western Blot
SuperSignal™
Horseradish Peroxidase
60 Mini-Gel Blots, 5000 sq. cm of membrane; 2 Mini-Gel Blots, 200 sq. cm of Membrane
Room Temperature
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For Research Use Only. Not for use in diagnostic procedures.