Invitrogen™ RecoverAll™ Multi-Sample RNA/DNA Isolation Workflow

Description

The RecoverAll™ Multi-Sample RNA/DNA Workflow is a complete workflow package for sequential extraction of RNA and DNA from the same formaldehyde- or paraformaldehyde-fixed paraffin-embedded (FFPE) tissue sample. The recovered RNA and DNA are ready for use in downstream applications such as quantitative real-time RT-PCR and targeted sequencing using the Oncomine™ Cancer Research Panel. This workflow package includes RecoverAll™ Total Nucleic Acid Isolation kits, elution tubes, and nuclease-free water.

Features include:
• Recovers RNA and DNA in separate eluates from the same FFPE section
• Relies on proven Ambion™ RecoverAll technology
• Processes four 20-μm sections per reaction

Special workflow provides more information from less sample input
FFPE samples are precious, which often requires the choosing of one extraction method over another to save sample. With the RecoverAll Multi-Sample RNA/DNA Workflow, recovering RNA and DNA from the same FFPE tissue sample in a single workflow eliminates the need to choose which nucleic acid to recover from a particular FFPE section.

First, FFPE slide-mounted or tissue blocks sections are deparaffinized and digested with protease. The samples are then loaded on a filter cartridge; DNA remains bound to the filter cartridge while RNA flows through. To recover RNA, the flow-through is applied to a new filter cartridge, treated with DNase, then eluted from the filter. To recover DNA, the DNA bound to the filter cartridge is treated with RNase A, then eluted from the filter.