Macherey-Nagel™ Protein Quantification Assay

Description

NucleoSpin™ miRNA is suitable for the parallel isolation of small RNA, large RNA and protein.

Superior selectivity - purify your RNA fractionated by size

• Isolation of small RNA only (18 to 200 bases)Isolation of small (18 to 200 bases) and large (>200 bases) RNA in two fractionsIsolation of total RNA (small and large in one fraction)

Additional isolation of total protein

• Denatured protein ready-to-use for analysis in SDS-PAGE and Western blot.

Excellent RNA recovery - even without phenol/chloroform

• Lysis with chaotropic saltSpin column based procedure

Convenient handling - complete equipment provided

• NucleoSpin filters - efficient sample homogenisationNucleoSpin protein removal columns - highest purity of small RNArDNase - highly efficient on-column digestion of genomic DNA at RT
• Silica membrane technology
• Format: minispin columns
• Sample size: <10⁷ cultured cells, <30mg human/tissue; <50 mg plant tissue, <150μL reaction mixture
• Fragment size, small RNA: 18 to 200 bases
• Fragment size, large RNA: >200 bases
• Typical yield: 10μg small RNA; 95μg large RNA from 10⁷ HeLa cells
• Binding capacity: 200μg
• Elution volume, 30μL to 100μL
• Preparation time: <45min (6 preps human/animal tissue, small and large RNA); <35min (6 preps human/animal tissue, small RNA only)
• Small RNA (18 to 200 bases)
• Large RNA (>200 bases)
• Total RNA (small and large RNA in one fraction), and isolate your total protein fraction For small and large RNA and total RNA purification, and isolation of total protein fraction.