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Thermo Scientific™ Pierce™ Quantitative Peroxide Assay Kit (Aqueous

Detect and measure hydrogen peroxide levels (H2O2) in biological samples using an iron (Fe) and xylenol orange (XO) reagent for microplates or tubes.

Brand:  Thermo Scientific™ 23280

236.55 EUR valid until 2024-11-29
Use promo code "21683" to get your promotional price.



Product Code. 10414195

  • 249.00€ / 1 set

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Description

Description

Detect and measure hydrogen peroxide levels in biological samples using an iron and xylenol orange reagent with Thermo Scientific™ Pierce Quantitative Peroxide Assay Kits.

These aqueous and lipid-compatible peroxide assay kits detect peroxides based on oxidation of ferrous to ferric ion in the presence of xylenol orange. One assay kit has an aqueous-compatible formulation that includes sorbitol, which provides sensitivity enhancement. The other assay kit has a formulation that may be used with lipid-containing samples without first extracting the lipids; however, because this kit does not contain sorbitol, the assay is less sensitive. Most proteins do not interfere with these assays, although some metal chelators may require use of a blank (i.e., control) to mitigate these effects.

In these assays, hydroperoxides convert the Fe2+ to Fe3+ at acidic pH. With the aqueous-compatible formulation, peroxide first reacts with sorbitol, converting it to a peroxyl radical, which in turn initiates Fe2+ oxidation to Fe3+. In the lipid compatible formulation, the peroxide converts the Fe2+ to Fe3+ directly. In a sulfuric acid solution, the Fe3+ complexes with the xylenol orange (XO) dye to yield a purple product having an absorbance maximum at 560nm. Peroxide levels in test samples can be determined by calculation from the know extinction coefficient of the XO-Fe complex or by reference to a standard curve prepared with hydrogen peroxide solution.

Highlights:

  • Peroxides – detects and measures hydrogen peroxide (H2O2) levels in biological and other liquids samples

  • Convenient – no proprietary reagents involved but the ready-to-use kits remove the variability, difficulty and added expense of formulating the reagents yourself

  • Versatile – quantitation by calculation from the extinction coefficient or by reference to a standard curve enables the method to be easily adapted to a variety of sample types and testing purposes

  • Two formulations – choose the aqueous-compatible kit for maximum sensitivity with non-lipid samples; choose the lipid-compatible kit for best results with lipid-containing samples

Recommended for:

General hydrogen peroxide (H2O2) measurement in protein samples; Quantitation of peroxides in detergents; Assessment of the effects of peroxides on cellular activity; Determination of protein glycation; Measurement of peroxide accumulation in lipids

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Sufficient For: 100 tube assays or 500 microplate assays with non-lipid samples
• Reagent A, 1 mL
• Reagent B, 2 x 50 mL

Store at 4°C.
Quantitative Peroxide Assay
Pierce™
Not Target-Specific
Solution-based Detection, Absorbance
Pierce Quantitative Peroxide Assay Kit (Aqueous)
Colorimetric
Quantitative Peroxide Assay
100 Tube Assays or 500 Microplate Assays
Spectrophotometer, Microplate Reader
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Safety and Handling

Safety and Handling

Product Identifier
  • Quantitative Peroxide Assay Kit

Signal Word
  • Danger

Hazard Category
  • Acute toxicity Category 3
  • Flammable liquid Category 2
  • Specific target organ toxicity after single exposure Category 1

Hazard Statement
  • H225-Highly flammable liquid and vapour.
  • H301-Toxic if swallowed.
  • H311-Toxic in contact with skin.
  • H331-Toxic if inhaled.
  • H370-Causes damage to organs.

Precautionary Statement
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P264-Wash hands thoroughly after handling.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P302+P352-IF ON SKIN: Wash with plenty of water/soap.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P308+P313-IF exposed or concerned: Get medical advice/attention.
  • P370+P378-In case of fire: Use dry sand, dry chemical or alcohol-resistant foam to extinguish.
  • P403+P233-Store in a well-ventilated place. Keep container tightly closed.
  • P403+P235-Store in a well-ventilated place. Keep cool.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information
  • MIXTURE LIST-Contains : methanol

SDS
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For Research Use Only. Not for use in diagnostic procedures.