Thermo Scientific™ Pierce™ Boronic Acid Resin

Description

Perform ribonucleotide and oligonucleotide RNA isolation with Thermo Scientific™ Pierce Boronic Acid Resin.

This immobilized boronic acid is an easy-to-use polyacrylamide affinity support for purification of ribonucleotides and other small molecular weight compounds that contain cis-diol groups. The ligand (m-aminophenyl-boronic acid) binds to the cis-diol groups on the sugar portion of nucleotides, forming a reversible five-member ring complex. After washing away non-bound molecules from the sample, the complex can be dissociated and the nucleotide eluted by lowering the pH or by addition of sorbitol.

Highlights:

• Ligand – boronic acid

• Support – beaded polyacrylamide (1800 MW exclusion limit)

• Spacer – m-aminophenyl group

• Loading – 100μmol boronate per milliliter of settled resin

• Capacity – at least 99% binding and recovery of 110μmol AMP per milliliter of settled resin

• Format – 50% resin slurry in 0.05% sodium azide