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Thermo Scientific™ Phusion High-Fidelity PCR Kit

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

Brand: Thermo Scientific™ F553S

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Product Code. 10044257

89.00€ / 50 reactions

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Description

Thermo Scientific Phusion High-Fidelity DNA Polymerase sets a high standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is an excellent choice for cloning and other applications requiring high fidelity. Phusion DNA polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerases.

The Phusion High-Fidelity PCR Kit contains all the necessary reagents for PCR including control lambda DNA template and primers for 1.3 kb and 10 kb amplicons. The DNA template amount is sufficient for performing 20 control reactions in 50 μL volume or 50 control reactions in 20 μL volume.

Highlights

High fidelity (52X Taq)
Fast PCR due to short extension times (15–30 s/kb)
Robust performance, minimal optimization needed
Increased product yields with minimal enzyme amounts

Applications

High-fidelity PCR
High throughput
Amplification of difficult (GC-rich) templates
Template generation for sequencing
Multiplex PCR
Long-range PCR
Cloning & mutagenesis
Microarray

Notes

Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).

Specifications

Content And Storage	• Phusion DNA Polymerase (2 U/μL) • 5X Phusion GC & HF Buffer • dNTP mix (10 mM each) • Control template (λ DNA) and DNA size standard • 1.3 kb & 10 kb primers (4 μM each) • 50 mM MgCl₂ solution and DMSO Store at –20°C.
Polymerase	Phusion High-Fidelity DNA Polymerase
Product Type	High-Fidelity PCR Kit
Shipping Condition	Dry Ice
Fidelity (vs. Taq)	52X
No. of Reactions	50 Reactions
Reaction Format	Separate Components

GC-Rich PCR Performance	High
Reaction Speed	Fast
Quantity	50 reactions
For Use With (Application)	Standard PCR, High-fidelity PCR
Hot Start	No
Overhang	Blunt
Size (Final Product)	20 kb or less

Product Suggestions Documents

Documents

Product Information

Manuals

User Guide: Phusion High-Fidelity PCR Kit Version: C.00 File size: 298.9 kB

Scientific Resources

Reference Materials

LabAid: Phusion High-Fidelity DNA Polymerases File size: 73.3 kB

Certificates

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Lot Number

5 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot Number2831439	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog NumberF553S
Lot Number2830843	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog NumberF553S
Lot Number2831991	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog NumberF553S
Lot Number2831990	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog NumberF553S
Lot Number2817400	Certificate TypeCertificate of Analysis	Date29/01/2025	Catalog NumberF553S

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