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Invitrogen™ LIVE/DEAD™ Cell Imaging Kit (488/570) Product Code.: 12353643

Invitrogen™ LIVE/DEAD™ Cell Imaging Kit (488/570)

Product Code. 12353643
1 mL kit, Each
Click to view available options
Quantity:
1 mL kit
Unit Size:
Each
This item is not returnable. View return policy
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”24113” to get your promotional price

Product Code. 12353643

Brand: Invitrogen™ R37601

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Edge
This item is not returnable. View return policy
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”24113” to get your promotional price

Includes

Component A: 10 x 1mL vials; Component B: 1 x 10 vials, dried down

Sensitive two-color fluorescence cell viability assay optimized for FITC and Texas Red™ filters

  • Allows discrimination between live and dead cells with two probes that measure recognized parameters of cytotoxicity and cell viability: intracellular esterase activity and plasma membrane integrity
  • Based on cell-permeable dye for staining of live cells and cell-impermeable dye for staining of dead and dying cells
  • Adapted for imaging platforms by optimizing for common green and red imaging filters used with FITC and Texas Red
  • Live cell component produces intense, uniform green fluorescence in live cells (ex/em 488 nm/515nm
  • Dead cell component produces predominantly nuclear red fluorescence (ex/em 570nm/602nm) in cells with compromised cell membranes, strong indicator of cell death and cytotoxicity
  • Sensitive detection of key measures of cell health: viability, live/dead ratio, and cytotoxicity
  • Ease of use with minimal dilutions
  • Live cells are distinguished by presence of ubiquitous intracellular esterase activity as determined by the enzymatic conversion of virtually nonfluorescent cell-permeant calcein AM to intensely fluorescent calcein, which is well-retained within live cells
  • Red component is cell-impermeant and therefore only enters cells with damaged membranes
  • In dying and dead cells bright red fluorescence is generated upon binding to DNA
  • Background fluorescence levels are inherently low with this assay technique because dyes are virtually nonfluorescent before interacting with cells

Cell Analysis, Cell Counting and Viability, Cell Counting, Viability, and Cryopreservation, Cell Culture, Cell Viability and Cytotoxicity, Cell Viability, Proliferation and Function

Order Info

Shipping Condition: Dry Ice

TRUSTED_SUSTAINABILITY

Specifications

Cell Type Mammalian Cells
Color Red, Green
Content And Storage • Component A: 10 x 1 mL vials
• Component B: 1 x 10 vials (dried down)

Store at -20°C
Description LIVE/DEAD™ Cell Imaging Kit (488/570)
Detection Method Fluorescence
For Use With (Application) Viability Assay
For Use With (Equipment) Floid Cell Imaging System, Fluorescence Microscope
Product Type Cell Imaging Kit
Dye Type Other Label(s) or Dye(s)
Emission 515/602
Excitation Wavelength Range 488, 570 nm
Form Frozen
Format Tube(s)
Incubation Time 15 min
Product Line LIVE/DEAD, Molecular Probes
Quantity 1 mL kit
Shipping Condition Dry Ice
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Certificates

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5 results found
Lot Number Certificate Type Date Product Code
Lot Number3079918 Certificate TypeCertificate of Analysis Date22/12/2024 Product CodeR37601_LITL18 PROMO,R37601
Lot Number2971024 Certificate TypeCertificate of Analysis Date11/07/2024 Product CodeR37601_LITL18 PROMO,R37601
Lot Number2925598 Certificate TypeCertificate of Analysis Date28/06/2024 Product CodeR37601_LITL18 PROMO,R37601
Lot Number2747522 Certificate TypeCertificate of Analysis Date09/01/2024 Product CodeR37601_LITL18 PROMO,R37601
Lot Number2743091 Certificate TypeCertificate of Analysis Date09/11/2023 Product CodeR37601_LITL18 PROMO,R37601
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