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Cytiva MBPTrap™ HP Prepacked Columns
Yield highly pure MBP-tagged recombinant proteins eluted in concentrated form and small volumes. Cytiva Life Sciences™ MBPTrap™ HP Prepacked Columns are prepacked with Dextrin Sepharose™ High Performance.
Brand: Cytiva 28-9187-80
This item is not returnable.
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Description
- Prepacked with Dextrin Sepharose tagged with maltose binding protein (MBP).
- Binding capacity is fusion protein dependent but can be as much as 16 mg/ml media
- Maximum pressure: 3 bar
- Compatible with commonly used buffers and easily regenerated with 0.5 M NaOH
- 1 and 5mL polypropylene columns can be used either manually using a syringe or on an instrument
Media
- Matrix: 6% cross-linked agarose
- Average particle size: 34μm
- pH stability working range: >7
1mL columns
- Bed dimensions: 7 x 25mm
- Flow velocity: <4mL/min.
5mL columns
- Bed dimensions: 16 x 25mm
- Flow velocity: <20mL/min.
Specifications
| Aqueous Buffer | |
| 1mL | |
| 16mm | |
| 0.98 in. | |
| <20mL/min. | |
| Dextrin | |
| >7 | |
| 6% Highly Cross-linked Agarose | |
| Ion Exchange Column |
| Dextrin Sepharose HP prepacked column | |
| 0.62 in. | |
| 5 x 5mL | |
| 25mm | |
| Polypropylene | |
| 3 bar | |
| 34μm | |
| 2° to 8°C | |
| Purification of MBP tagged Proteins |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- MBPTrap HP
Signal Word
- Warning
Hazard Category
- Flammable liquid Category 3
Hazard Statement
- H226-Flammable liquid and vapour.
Precautionary Statement
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P241-Use explosion-proof electrical/ventilating/lighting/and all material-handling/ equipment.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P235-Keep cool.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : ethanol