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Thermo Scientific™ Kinase Tracer 236, 50 μM

In the LanthaScreen™ Eu Kinase Binding Assay, binding of an Alexa Fluor™ conjugate or tracer such as Kinase Tracer 236 to a kinase is detected by addition of a Eu-labeled anti-tag antibody.

Brand:  Thermo Scientific™ PV5592

Product Code. 10529763

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Description

Description

In the LanthaScreen™ Eu Kinase Binding Assay, binding of an Alexa Fluor™ conjugate or tracer such as Kinase Tracer 236 to a kinase is detected by addition of a Eu-labeled anti-tag antibody. Binding of the tracer & antibody to a kinase results in a high degree of FRET, whereas displacement of the tracer with a kinase inhibitor results in a loss of FRET. Unlike many kinase activity assays, this assay is a simple mix and read assay, with no development steps.

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Kinase Binding Assay
Store at -20°C.
Microplate Reader
Alexa Fluor™ 647
Kinetic
CSF1R, CSK, EPHA5, PDGFRA T674I, PDGFRA V561D, RET Y791F, SYK, TYRO3, ZAP70, MAP4K5, PRKCQ, PRKX, ROCK2, IKBKE, PAK4, MAP3K2, LRRK2 D1994A, GRK7, GSK3A, GSK3B, SNF1LK2, MAP2K1 S218D S222D, FGFR1, FGFR2, MAP3K10, MAP3K9, JAK2 JH1 JH2, AMPK (A2/B1/G1), CAMK2B, CHEK1, PIM2, PRKCN, PRKD2, PRKG2, RPS6KA5, MELK, BLK, EPHB2, IGF1R, LYNA, MERTK, RET V804L, ROS1, TEK, TTK, SGK, CHUK, IKBKB, TAOK3, TYK2, IRAK1, MYLK2, SRPK2, STK4, FLT1, MUSK, CAMK2D, LRRK2 R1441C, DYRK1A, DYRK3, MAP2K1, MAP2K6, RPS6KA6, BMX, EPHA4, FYN, PTK2, PTK2B, RET, MAP4K2, MINK1, GRK6, JAK3, LRRK2, AMPK (A1/B1/G1), AURKA, CAMK1D, CAMK4, PIM1, PKN1, PRKD1, PRKG1, RPS6KA4, STK22D, NUAK1, EPHA3, FLT3, FLT4, INSR, LCK, YES1, KDR, MET, TNK2, PRKCI, CAMK2A, RPS6KA3, MAP3K3, ABL2, AXL, FGFR3, FGFR3 K650E, FGR, HCK, AURKB, TAOK2, MAP3K11, CAMKK1, CDC42BPA, CDK1, CDK5, CDK8, CDK9/Cyclin K, MAP2K3, CSNK1G3, DMPK, DYRK1B, ACVR1, EPHA1, EPHA8, LYNB, MST1R, PDGFRA D842V, MAP4K4, LRRK2 G2019S, CAMKK2, CDC42BPB, CDK2, CDK7, CDK9/Cyclin T1, EPHA2, FER, LTK, PDGFRA, PDGFRB, MARK4, RPS6KB1, EGFR T790M, EPHA7, EPHB1, PHKG1, ROCK1, RPS6KA1, TBK1, JAK2, MARK1, PHKG2, MLCK, PAK6, SLK, LIMK1, MKNK2, MYLK, FRK, ITK, LRRK2 I2020T, BRSK1, CLK2, PDK1, MAP2K2, MAP3K14, MAP2K6 S207E T211E, FLT3 D835Y, SRC, GRK5, GRK4, STK3, BTK, FES, KIT T670I, RPS6KA2, SGKL, STK25, PAK7, PRKACA, CLK1, CLK4, MARK2, STK17A, PRKCH, EIF2AK2, MAP3K5, LIMK2, STK16
Biochemical binding/kinetic
TR-FRET
384-well Plate
25 μL
Dry Ice
Kinase Tracer
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