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Thermo Scientific™ Kinase Tracer 178, 25 μM
In the LanthaScreen™ Eu Kinase Binding Assay, binding of an Alexa Fluor™ conjugate or tracer such as Kinase Tracer 178 to a kinase is detected by addition of a Eu-labeled anti-tag antibody.
Brand: Thermo Scientific™ PV5593
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Description
In the LanthaScreen™ Eu Kinase Binding Assay, binding of an Alexa Fluor™ conjugate or tracer such as Kinase Tracer 178 to a kinase is detected by addition of a Eu-labeled anti-tag antibody. Binding of the tracer & antibody to a kinase results in a high degree of FRET, whereas displacement of the tracer with a kinase inhibitor results in a loss of FRET. Unlike many kinase activity assays, this assay is a simple mix and read assay, with no development steps.
Specifications
| Microplate Reader | |
| Kinases (Multiple) | |
| Kinase Binding Assay | |
| Biochemical binding/kinetic | |
| Kinase Tracer | |
| 384-well plate | |
| Dry Ice |
| LanthaScreen™ | |
| TR-FRET | |
| Store at -20°C. | |
| Alexa Fluor 647 | |
| 25 μL | |
| Kinetic | |
| PTK6, DDR2, BMPR1A, RAF1 (cRAF) Y340D Y341D, ACVR1B, WNK2, TEC, DDR1, EPHB4, ERBB4, KIT V654A, TGFBR1, NLK, TGFBR2, EPHB3, BRAF V599E, BRAF, ACVR2B, ACVR2A |
For Research Use Only. Not for use in diagnostic procedures.