Invitrogen™ Western Devices Benchtop Bundle

Description

The Western Devices Benchtop Bundle contains all the necessary devices, consumables, and reagents to perform high quality westerns.

Contents of the Western Devices Benchtop Bundle:
• Mini Gel Tank (Cat. No. A25977)
• Bolt 4-12% Bis-Tris Plus gels, 10-well (Cat. No. NW04120BOX)
• Bolt MES Running Buffer, 20X (Cat. No. B0002)
• Bolt LDS Sample Buffer, 4X (Cat. No. B0007)
• Bolt Sample Reducing Agent, 10X (Cat. No. B0009)
• SeeBlue Plus2 Pre-stained Protein Standard (Cat. No. LC5925)
• iBlot 2 Gel Transfer Device (Cat. No. IB21001)
• iBlot 2 Transfer Stacks, nitrocellulose, regular size (Cat. No. IB23001)
• iBind Western Device (Cat. No. SLF1000)
• iBind Cards (Cat. No. SLF1010)
• iBind Solution Kit (Cat. No. SLF1020)
• SuperSignal West Pico Plus Chemiluminescent Substrate (Cat. No. 34577)

Mini Gel Tank
The Mini Gel Tank is compatible with Novex gels, NuPAGE gels, and Bolt Bis-Tris Plus gels. Each Mini Gel Tank can accommodate up to two gels per run. The unique tank design enables convenient side-by-side gel loading and enhanced viewing during use. Optimized conditions using constant voltage allow for Bolt Bis-Tris Plus gels to be run in approximately 35 minutes.

Bolt Bis-Tris Plus gels
Bolt Bis-Tris Plus gels are precast polyacrylamide gels designed for optimal separation of a wide range of protein sizes under denaturing conditions. Bolt Bis-Tris Plus gels are designed to deliver consistent gel performance and provide a neutral pH environment that minimizes protein modifications. Bolt gels are ideal for western blot transfer and analysis, and any other techniques where protein integrity is crucial. Also use Bolt gels to obtain optimal results for your day-to-day protein separation needs.

iBlot 2 Gel Transfer Device
The iBlot 2 Gel Transfer Device performs western blotting transfer simply, efficiently, and reliably, within seven minutes without the need for liquid buffers. The iBlot 2 system is compatible with both polyvinylidene difluoride (PVDF) and nitrocellulose membranes, and has comparable performance to traditional wet transfer methods in a fraction of the time. The iBlot 2 Gel Transfer Device is an integral part of the iBlot 2 Dry Blotting system, which consists of the transfer device and consumable transfer stacks that contain the required buffers and transfer membrane.

iBind Western Device
The iBind Western Device is an automated western-processing device that performs every step in the western protocol from blocking to washes to antibody incubations via sequential lateral flow (SLF). SLF allows the timely release and flow of solutions and antibodies to the membrane without need of an external power source. Each solution is released from the iBind device wells to an iBind Card via SLF; the solutions are then wicked towards the stack region of the card. The glass fiber matrix of the card allows for homogenous and consistent flow of the solutions to the membrane, increasing the antigen-antibody interaction. The iBind western detection procedure minimizes hands-on time and is highly reproducible and compatible with all western protocols. Set up time is fast: in less than 3 hours you are ready for the detection step.