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Invitrogen™ Proteinase K Solution (20 mg/mL), RNA grade
Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins.
Brand: Invitrogen™ 25530049
254.10 EUR valid until 2024-11-30
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This item is not returnable.
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Description
Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins.
- Over a wide pH range—optimal activity between 6.5 and 9.5
- Under denaturing conditions—e.g., in the presence of SDS or urea
- In the presence of metal chelating agents—e.g., EDTA
- At comparatively high temperatures—optimum digestion temperature is 65°C
- Protein Filter: P
Applications
Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization.
Performance and quality testing
Endodeoxyribonuclease and exodeoxyribonuclease assays; liquid form tested for absence of RNase activity.
Unit definition
One mAnson unit is described as that amount of enzyme that liberates 1 μmol of Folin-positive amino acid within one minute at 37°C using hemoglobin as a substrate.
Specifications
| 20 mg/mL | |
| Chromatin Biology/RNA Extraction/DNA Extraction | |
| Approved for shipment on Wet or Dry Ice | |
| Proteinase K |
| DNase-Free, RNase-Free | |
| Store in freezer (-5 to -30°C). | |
| 5 mL | |
| Liquid |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- 5 ML PROTEINASE K SOLUTION RNA STORE AT -20C
Signal Word
- Danger
Hazard Category
- Respiratory sensitiser Category 1
Hazard Statement
- H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
Precautionary Statement
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P284-¢In case of inadequate ventilation! wear respiratory protection.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : Proteinase, tritirachium album serine
For Research Use Only. Not for use in diagnostic procedures.