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Invitrogen™ pBAD TOPO™ TA Expression Kit

Specifically designed for one-step cloning and regulated prokaryotic expression of Taq-amplified PCR products

1206.00€ - 2095.00€

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Specifications

Constitutive or Inducible System	Inducible
Inducing Agent	Arabinose
Promoter	araBAD
Product Type	TA Expression Kit
Selection Agent (Eukaryotic)	None

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Includes: Each pBAD TOPO TA Expression Kit contains two boxes and the LMG194 E. coli stab. The pBAD TOPO TA box contains all of the reagents necessary for PCR (except Taq polymerase), including 200ng of topoisomerase I-activated pBAD-TOPO vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control template and primers, 20% L-arabinose, pBAD primers for sequencing or PCR screening, and an expression control plasmid. The other box contains all of the reagents necessary for transformation, including single-use 50μL aliquots of TOP10 Chemically Competent E. coli, S.O.C. medium, and a supercoiled control plasmid.

Products 2

Product Code	Brand	Protein Tag	Quantity	Price	Quantity & Availability
Product Code	Brand	Protein Tag	Quantity	Price	Quantity & Availability
10414592 View Documents Promotion Details missing translation for 'viewProductCertificates'	Invitrogen™ K430040	V5 Epitope Tag	40 Reactions	2095.00€ Each		Please sign in to purchase this item. Need a web account? Register with us today!



10115092 View Documents missing translation for 'viewProductCertificates'	Invitrogen™ K430001	His Tag (6x), V5 Epitope Tag	20 Reactions	1206.00€ Each		Please sign in to purchase this item. Need a web account? Register with us today!

Description

Linearized, topoisomerase I-activated vector for 5-minute cloning of Taqamplified PCR products
araBAD promoter for tightly regulated expression in E. coli V5 epitope tag for detection with Anti-V5 Antibody
C-terminal polyhistidine (6xHis) tag for purification using nickel-chelating resin and detection with Anti-His(C-term) Antibody
Enterokinase cleavage site for removal of N-terminal leader peptide

Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Specifications

Constitutive or Inducible System	Inducible
Promoter	araBAD
Selection Agent (Eukaryotic)	None
Bacterial or Yeast Strain	LMG194, TOP10
Expression System	E. coli
Cloning Method	TOPO-TA
Product Line	TOPO™

Inducing Agent	Arabinose
Product Type	TA Expression Kit
Antibiotic Resistance Bacterial	Ampicillin (AmpR)
Cleavage	EK (Enterokinase) Recognition Site
Expression Mechanism	Cell-Based Expression
Vector	pBAD

For Research Use Only. Not for use in diagnostic procedures.

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Invitrogen™ pBAD TOPO™ TA Expression Kit

Specifications

Description

Specifications

Documents