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Invitrogen™ pBAD TOPO™ TA Expression Kit
Specifically designed for one-step cloning and regulated prokaryotic expression of Taq-amplified PCR products
1206.00€ - 2095.00€
Specifications
| Constitutive or Inducible System | Inducible |
|---|---|
| Inducing Agent | Arabinose |
| Promoter | araBAD |
| Product Type | TA Expression Kit |
| Selection Agent (Eukaryotic) | None |
Includes: Each pBAD TOPO TA Expression Kit contains two boxes and the LMG194 E. coli stab. The pBAD TOPO TA box contains all of the reagents necessary for PCR (except Taq polymerase), including 200ng of topoisomerase I-activated pBAD-TOPO vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control template and primers, 20% L-arabinose, pBAD primers for sequencing or PCR screening, and an expression control plasmid. The other box contains all of the reagents necessary for transformation, including single-use 50μL aliquots of TOP10 Chemically Competent E. coli, S.O.C. medium, and a supercoiled control plasmid.
| Product Code | Brand | Protein Tag | Quantity | Price | Quantity & Availability | ||||
|---|---|---|---|---|---|---|---|---|---|
| Product Code | Brand | Protein Tag | Quantity | Price | Quantity & Availability | ||||
|
10414592
|
Invitrogen™
K430040 |
V5 Epitope Tag | 40 Reactions |
2095.00€
Each |
Please sign in to purchase this item. Need a web account? Register with us today! | ||||
|
10115092
|
Invitrogen™
K430001 |
His Tag (6x), V5 Epitope Tag | 20 Reactions |
1206.00€
Each |
Please sign in to purchase this item. Need a web account? Register with us today! | ||||
Description
- Linearized, topoisomerase I-activated vector for 5-minute cloning of Taqamplified PCR products
- araBAD promoter for tightly regulated expression in E. coli V5 epitope tag for detection with Anti-V5 Antibody
- C-terminal polyhistidine (6xHis) tag for purification using nickel-chelating resin and detection with Anti-His(C-term) Antibody
- Enterokinase cleavage site for removal of N-terminal leader peptide
Bacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis
Specifications
| Inducible | |
| araBAD | |
| None | |
| LMG194, TOP10 | |
| E. coli | |
| TOPO-TA | |
| TOPO™ |
| Arabinose | |
| TA Expression Kit | |
| Ampicillin (AmpR) | |
| EK (Enterokinase) Recognition Site | |
| Cell-Based Expression | |
| pBAD |
For Research Use Only. Not for use in diagnostic procedures.