Invitrogen Platinum SuperFi PCR Master Mix is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. Only template, primers, and water need to be added, thus reducing the number of pipetting steps during PCR reaction setup.
Platinum SuperFi PCR Master Mix retains all the features of Platinum SuperFi DNA Polymerase, a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology. Platinum SuperFi DNA Polymerase is ideally suited for cloning, mutagenesis, and other applications benefiting from supreme sequence accuracy.
Platinum SuperFi DNA Polymerase is engineered with a DNA-binding domain resulting in high processivity and increased resistance to PCR inhibitors. This feature also enables fast-cycling protocols and amplification of long targets. The Platinum hot-start technology is based on proprietary antibodies that inhibit enzyme activity until the initial PCR denaturation step, preventing non-specific amplification and primer degradation.
Features
Exceptional >300X Taq fidelity
High specificity and increased yields with Platinum hot-start technology
Robust amplification of difficult targets of high GC content
Room temperature reaction setup and 24-hour bench top stability of pre-assembled reactions
Amplification of samples with suboptimal purity
Applications
High-fidelity PCR
Cloning and sub-cloning
Site-directed mutagenesis
Amplification of GC-rich templates
Template generation for sequencing
High-throughput PCR
Long PCR
Notes
Annealing rules for Platinum SuperFi DNA Polymerase are different from many common DNA polymerases.
Platinum SuperFi PCR Master Mix is supplied with separate SuperFi GC Enhancer for difficult and GC-rich templates (˃65% GC).
