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Invitrogen™ eBioscience™ Intracellular Fixation & Permeabilization Buffer Set

Designed for use in intracellular staining and flow cytometric analysis and has been specially formulated to reduce non-specific staining of fluorochrome-labelled antibodies and increase fluorescence signal-to-noise ratios.

Brand:  Invitrogen™ 88-8824-00

136.00 EUR valid until 2025-03-29
Use promo code "24111" to get your promotional price.


Product Code. 15284728

  • 204.00€ / Each

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Alert:

Contains Sodium azide and formaldehyde

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To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”24111” to get your promotional price


Includes:
  • IC Fixation Buffer
  • Permeabilization Buffer (10X)
This item is not returnable. View return policy

Description

Description

  • In the first step, live cells can be 'fixed' with the Fixation Buffer, which cross- links proteins
  • The second step, using the Permeabilization Buffer, creates holes in the membrane and allows the intracellular staining antibodies to enter the cell effectively
  • Subsequent washing steps, antibody additions, and incubations after cell permeabilization should be performed using the Permeabilization Buffer
  • Final washing step and cell resuspension prior to running the sample is done using Flow Cytometry Staining Buffer (cat. 00-4222).
  • This kit is commonly used to stain cytoplasmic proteins and proteins in the secretory pathway such as chemokines and cytokines
  • Not recommended for intranuclear staining. Instead, please refer to the Foxp3/Transcription Factor Buffer Set for nuclear staining
  • Before the fixation step, it is recommended to label the live cells using one of the Fixable Viability Dyes and then proceed with fixation
TRUSTED_SUSTAINABILITY
Specifications

Specifications

eBioscience™
Fixn/Perm Buffer Set
1 kit
Fixation/Permeabilization Buffer
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For Research Use Only. Not for use in diagnostic procedures.