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Cytiva HiTrap™ Chelating HP Columns

Optimize histidine-tagged protein purifications when nickel ions are not the best choice. Cytiva Life Sciences™ HiTrap™ Chelating HP Columns is prepacked with Chelating Sepharose™ High Performance.

Brand: Cytiva 17-0409-05

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Product Code. 11850073

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Description

Uses different kinds of metal ions
Binding capacity ∽12mg histidine-tagged protein/mL medium
Metal ion capacity ∽23μmol Cu²⁺/mL medium
Prepacked with Chelating Sepharose High Performance media
Iminoacetic acid coupled to a 6% agarose matrix by ether linkage

Specifications

Buffer	6M Guanidine Hydrochloride, 8M Urea
Bed Volume	5mL
Height (English)	0.98 in.
Flow Rate	<20 mL/min.
Ligand Type	Iminodiacetic Acid
pH Range	3 to 13
Support Type	6% Highly Cross-linked Agarose
Column Type	Affinity Column

Product Type	HiTrap chelating HP column
Quantity	100 x 5 mL
Height (Metric)	25mm
Material	Iminiodiacetic acid chelating group
Max. Pressure	3 bar
Particle Size	34μm
Content And Storage	4° to 30°C
For Use With (Application)	IMAC and subsequent purification of Polyhistidine tagged Proteins

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Cytiva HiTrap™ Chelating HP Columns

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