Molecular Probes™ HCS NuclearMask™ Stains

Description

Easily identify cell nuclei in high-content screening (HCS) assays with the versatile HCS NuclearMask Blue, Red, and Deep Red stains, which survive standard formaldehyde-based fixation and detergent-based permeabilization methods and can be applied to live and fixed cells. Because their dyes are easily discriminated from other fluorophores, the HCS NuclearMask stains can be used in multiparametric analyses. They can also label the entire cell (i.e., cytoplasm and nucleus) and provide an accurate backdrop against which to assess the features of interest.

The versatile HCS NuclearMask Blue, HCS NuclearMask Red, and HCS NuclearMask Deep Red stains can be used to measure DNA content and perform cell demarcation with formaldehyde-fixed cells on high content imaging and analysis (HCS) platforms. HCS NuclearMask Blue, HCS NuclearMask Red, and HCS NuclarMask Deep Red stains have excitation/emission maxima of 355/460 nm, 622/645 nm, and 638/686 nm, respectively, when bound to DNA. HCS NuclearMask Blue stain is easily discriminated from visible- and near-IR wavelength fluorophores, HCS NuclearMask Red stain is easily discriminated from green- and even orange-fluorescent fluorophores, and HCS NuclearMask Deep Red is easily discriminated from visible wavelength fluorophores when used in multiparametric analyses.

If total cell staining is desired, HCS CellMask stains label the entire cell (cytoplasm and nucleus) and provide an accurate backdrop against which the features of interest can be assessed. HCS CellMask stains are available in a choice of colors for multiplexing flexibility. Sufficient volume quantities are provided to stain ten 96-well plates using an assay volume of 100 μL per well.