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Thermo Scientific™ GeneBLAzer™ RXR beta DA Assay Kit

Brand: Thermo Scientific™ K1405

Product Code. 10638723

1586.00€ / Each

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Description

GeneBLAzer™RXR beta DA (Division Arrested) cells and RXR beta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Retinoid X Receptor beta (RXR beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line. GeneBLAzer™UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-RXR beta (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both RXR beta DA cells and RXR beta-UAS-bla HEK 293T cells are functionally validated for Z' and EC₅₀ concentrations of 9-cis retinoic acid (9-cis-RA). In addition, RXR beta-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, and stimulation time.

Specifications

Alternate Agonists	Progesterone
Assay Entry	Cell-based beta lactamase reporter gene
Content And Storage	Contains sufficient division-arrested cells and substrate to assay 1 x 384-well plate: • RXR beta HEK 293T DA cells (K1405A) • LiveBLAzer™-FRET B/G Loading Kit, 70 μg Shipping: Dry Ice Storage: -80°C (liquid nitrogen)
Detection Method	Fluorescence
Gene Symbol	RXRβ
Primary Agonist	R5020
Quantity	1 Plate
Sample Type	293T (HEK)
Type	Division-Arrested Cells Assay Kit
System Type	GeneBLAzer™

Antagonists	RU 486
Cell State	Division-Arrested Cells
Description	GeneBLAzer™ RXR beta DA Assay Kit, 1Plate, ∽ 4,800,000 cells/vial Quantity of Cells, 293T (HEK) Cell Line, Fluorescent Detection Method, Nuclear receptors Druggable Target, BLA (Beta-Lactamase) Reporter, Adherent Growth Properties, –80°C/Liquid Nitrogen Storage
For Use With (Application)	Cellular imaging, pharma and biopharma, target and lead identification and validation, target-based adme/tox assays, Cell analysis, cell-based reporter assays, cell-based and lactamase assays, drug discovery and development, nuclear receptor biology, cell-based nuclear receptor reporter assays, target-based ADME/tox assays, cellular adme assays, nuclear receptor cellular adme assays
Includes	RXR beta HEK 293T DA cells (K1405A) LiveBLAzer™-FRET B/G Loading Kit, 70μg
Product Line	GeneBLAzer™
Reporter Gene	BLA (Beta-Lactamase)
Target Entry	RXR beta, NR2B2, RXRB
Cell Line	293T (HEK)
Druggable Target	Nuclear Receptors

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Documents

Product Information

Manuals

User Guide: GeneBLAzer RXR beta HEK 293T DA and RXR beta-UAS-bla HEK 293T Cell-based Assay Version: A.0 File size: 277.4 kB

GeneBLAzer® RXR beta Validation Packet Version: version 1.1 File size: 237.9 kB

Certificates

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Lot Number

2 results found

Lot Number	Certificate Type	Date	Catalog Number
Lot Number374331	Certificate TypeCertificate of Analysis	Date15/04/2017	Catalog NumberK1405
Lot Number885120	Certificate TypeCertificate of Analysis	Date15/04/2017	Catalog NumberK1405

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