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Thermo Scientific™ SP6 RNA Polymerase (20 U/μL)
Thermo Scientific™ SP6 RNA Polymerase is a DNA-dependent RNA polymerase with strict specificity for its double-stranded promoter.
Brand: Thermo Scientific™ EP0131
66.21 EUR valid until 2025-03-28
Use promo code "24107" to get your promotional price.
Description
Thermo Scientific™ Bacteriophage SP6 RNA polymerase is DNA-dependent RNA polymerase with strict specificity for their respective double-stranded promoters.
It catalyzes the 5'=>3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from it promoter.- Incorporates modified nucleotides (e.g., aminoallyl-, biotin-, fluorescein-, digoxigenin-labeled nucleotides)
Recommended for:
Synthesis of unlabeled and labeled RNA that can be used: for hybridization (1), in vitro RNA translation (2); as aRNA (3), siRNA (4), substrate in RNase protection assays (5), template for genomic DNA sequencing.
Note:
Consensus promoter sequences: AATTAACCCTCACTAAA G GGAGA - T3 TAATACGACTCACTATA G GGAGA - T7 ATTTAGGTGACACTATA G AAGNG - SP6 The position in bold (+1) indicates the first nucleotide incorporated into RNA during transcription. Only bases at this position through +3 are critical for transcription, and they must be a G and a purine base, respectively (9).
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Specifications
SP6 RNA Polymerase | |
2,000 units | |
1X |
Certificates
A lot number is required to show results for certificates. To find your lot number on previous orders use our order status area.
Lot Number | Certificate Type | Date | Catalog Number |
---|---|---|---|
2845858 | Certificate of Analysis | 28/01/2025 | C22322.30 |
2845847 | Certificate of Analysis | 28/01/2025 | C22322.30 |
2872536 | Certificate of Analysis | 28/01/2025 | EP0131 |
2888380 | Certificate of Analysis | 28/01/2025 | EP0131 |
2920942 | Certificate of Analysis | 27/01/2025 | EP0131 |
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