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Thermo Scientific™ Genomic DNA Purification Kit
Isolate genomic DNA from various sources, including whole blood, serum, cell lines, bacterial cells, plant, and mammalian tissues, with this DNA precipitation system.
Brand: Thermo Scientific™ K0512
107.90 EUR valid until 2024-11-30
Use promo code "21651" to get your promotional price.
This item is not returnable.
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Description
Genomic DNA Purification Kit is a simple and rapid system for high quality genomic DNA purification from various sources including: whole blood, serum, cell lines, bacterial cells, plant, and mammalian tissues.
The kit is based on selective detergent-mediated DNA precipitation from crude lysate. The entire procedure is rapid only 20 to 25 minutes with a typical yield of 2 to 10 μg genomic DNA from 0.2 mL of blood.
High molecular weight genomic DNA purified with the kit is suitable for direct use in all common molecular biology applications: PCR, FastDigest and conventional restriction digestion, cloning, DNA sequencing, and Southern blot analysis.
Highlights
- Efficient—2 to 10 μg of genomic DNA from 0.2 mL of blood
- Fast—procedure takes approximately 20 minutes
- Universal—purifies genomic DNA from various sources
- Flexible—easy to scale up and down
- Safe—does not involve phenol extraction
Specifications
| • 40 mL Lysis Solution; room temperature • 8 mL Precipitation Solution (concentrated); room temperature • 10 mL NaCl Solution; room temperature |
|
| Bacteria, Cells, Plant, Serum, Tissue, Whole Blood, Blood | |
| gDNA | |
| Not High-throughput Compatible (Manual) | |
| Room Temperature | |
| Bacteria: ≤20 mg Cells: ≤106 Plant: ≤100 mg Tissue: ≤30 mg Serum: ≤200 μL Whole Blood: ≤200 μL |
| Organic Extraction | |
| 20 to 100 μL | |
| PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization | |
| 100 Preps | |
| 25 min. |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Genomic DNA Purification Kit
Signal Word
- Danger
Hazard Category
- LONG-TERM AQUATIC HAZARD Chronic 2
- ACUTE AQUATIC Acute 1
- Serious eye damage/eye irritation Category 1
- Skin corrosion/irritation Category 2
- Specific target organ toxicity after repeated exposure Category 2
- Specific target organ toxicity Category 3
Hazard Statement
- H315-Causes skin irritation.
- H318-Causes serious eye damage.
- H335-May cause respiratory irritation.
- H373-May cause damage to organs.
- H400-Very toxic to aquatic life.
- H411-Toxic to aquatic life with long lasting effects.
Precautionary Statement
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P264-Wash hands thoroughly after handling.
- P271-Use only outdoors or in a well-ventilated area.
- P273-Avoid release to the environment.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P310-Immediately call a POISON CENTER/doctor/.
- P312-Call a POISON CENTER/doctor/if you feel unwell.
- P314-Get medical advice/attention if you feel unwell.
- P332+P313-If skin irritation occurs: Get medical advice/attention.
- P391-Collect spillage.
- P403+P233-Store in a well-ventilated place. Keep container tightly closed.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : N,N,N-trimethyl-1-tetradecanaminium bromide, Hexadecyltrimethylammonium bromide
For Research Use Only. Not for use in diagnostic procedures.