CD194 (CCR4) Monoclonal Antibody (D8SEE), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Description

Description: This D8SEE monoclonal antibody reacts with human CD194 (CCR4). CD194, also known as C-C Chemokine Receptor 4 (CCR4) is a member of the G protein-coupled receptor family, a large family characterized by seven transmembrane alpha helical domains. The ligands for CCR4 are CCL17 (TARC) and CCL22 (MDC), which direct the migration of target cells. CCR4 and its ligands, along with the other receptors CCR10 and CLA, are particularly important for the recruitment of T cells into the skin, where they may also play a role in adhesion and extravasation. Although CCR4 expression was originally believed to be restricted to Th2 cells, it has since been observed in some subsets of Th17, Treg, and Th1 cells. It is not expressed by naive T cells, indicating a role in memory or effector function. Other cells that express CCR4 are activated NK cells, platelets, monocytes, and basophils. Applications Reported: This D8SEE antibody has been reported for use in flow cytometric analysis. Applications Tested: This D8SEE antibody has been pre-titrated and tested by flow cytometric anaylis of normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.